ArticleCell host & microbe2019
Spacer Acquisition Rates Determine the Immunological Diversity of the Type II CRISPR-Cas Immune Response.
Article in Cell host & microbe, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Single nucleotide variants drive evolutionary phage-host arms race in anaerobic carbon dioxide-converting microbiome.Nature communications · 2026Article
- CRISPR-Cas spacer acquisition is a rare event in human gut microbiome.Cell genomics · 2025Article
- A novel genus of Pectobacterium bacteriophages display broad host range by targeting several species of Danish soft rot isolates.Virus research · 2024Article
- My host's enemy is my enemy: plasmids carrying CRISPR-Cas as a defence against phages.Proceedings. Biological sciences · 2024Article
- Assessing spacer acquisition rates inFrontiers in microbiology · 2024Article
- Rarely acquired type II-A CRISPR-Cas spacers mediate anti-viral immunity through the targeting of a non-canonical PAM sequence.Nucleic acids research · 2023Article
- CRISPR arrays as high-resolution markers to track microbial transmission during influenza infection.Microbiome · 2023Article
- Unveil the Secret of the Bacteria and Phage Arms Race.International journal of molecular sciences · 2023Review
- Article
- Epidemiological and evolutionary consequences of different types of CRISPR-Cas systems.PLoS computational biology · 2022Article
- Cleavage of viral DNA by restriction endonucleases stimulates the type II CRISPR-Cas immune response.Molecular cell · 2022Article
- Immune lag is a major cost of prokaryotic adaptive immunity during viral outbreaks.Proceedings. Biological sciences · 2021Article
- Viral recombination systems limit CRISPR-Cas targeting through the generation of escape mutations.Cell host & microbe · 2021Article
- Survival Strategies ofViruses · 2021Article
- It is unclear how important CRISPR-Cas systems are for protecting natural populations of bacteria against infections by mobile genetic elements.Proceedings of the National Academy of Sciences of the United States of America · 2020Review
- Article
- Cas9 Cleavage of Viral Genomes Primes the Acquisition of New Immunological Memories.Cell host & microbe · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
CRISPR-Cas systems provide acquired immunity in prokaryotes. Upon infection, short sequences from the phage genome, known as spacers, are inserted between the CRISPR repeats. Spacers are transcribed into small RNA molecules that guide nucleases to their targets. The forces that shape the distribution of newly acquired spacers, which is observed to be uneven, are poorly understood. We studied the spacer patterns that arise after phage infection of Staphylococcus aureus harboring the Streptococcus pyogenes type II-A CRISPR-Cas system. We observed that spacer patterns are established early during the CRISPR-Cas immune response and correlate with spacer acquisition rates, but not with spacer targeting efficiency. The rate of spacer acquisition depended on sequence elements within the spacer, which in turn determined the abundance of different spacers within the adapted population. Our results reveal how the two main forces of the CRISPR-Cas immune response, acquisition and targeting, affect the generation of immunological diversity.
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