ArticleMolecular cell2019
A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
Article in Molecular cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Insights into spacer acquisition of the type V-A CRISPR-Cas system of Francisella novicida U112.Nucleic acids research · 2026Article
- Structural biology of CRISPR-Cas immunity and genome editing enzymes.Nature reviews. Microbiology · 2022Review
- Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types.The CRISPR journal · 2022Article
- Protein PEGylation: Navigating Recombinant Protein Stability, Aggregation, and Bioactivity.BioMed research international · 2022Review
- Sequence specific integration by the family 1 casposase from Candidatus Nitrosopumilus koreensis AR1.Nucleic acids research · 2021Article
- Article
- Allosteric regulation in CRISPR/Cas1-Cas2 protospacer acquisition mediated by DNA and Cas2.Biophysical journal · 2021Article
- Structural coordination between active sites of a CRISPR reverse transcriptase-integrase complex.Nature communications · 2021Article
- Robust direct digital-to-biological data storage in living cells.Nature chemical biology · 2021Article
- Genomic Analysis of Molecular Bacterial Mechanisms of Resistance to Phage Infection.Frontiers in microbiology · 2021Article
- A scoutRNA Is Required for Some Type V CRISPR-Cas Systems.Molecular cell · 2020Article
- Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas.eLife · 2020Article
- Fidelity of prespacer capture and processing is governed by the PAM-mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system.The Journal of biological chemistry · 2019Article
- CRISPR DNA elements controlling site-specific spacer integration and proper repeat length by a Type II CRISPR-Cas system.Nucleic acids research · 2019Article
- CRISPR repeat sequences and relative spacing specify DNA integration by Pyrococcus furiosus Cas1 and Cas2.Nucleic acids research · 2019Article
- Sequence motifs recognized by the casposon integrase of Aciduliprofundum boonei.Nucleic acids research · 2019Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
CRISPR-Cas immunity requires integration of short, foreign DNA fragments into the host genome at the CRISPR locus, a site consisting of alternating repeat sequences and foreign-derived spacers. In most CRISPR systems, the proteins Cas1 and Cas2 form the integration complex and are both essential for DNA acquisition. Most type V-C and V-D systems lack the cas2 gene and have unusually short CRISPR repeats and spacers. Here, we show that a mini-integrase comprising the type V-C Cas1 protein alone catalyzes DNA integration with a preference for short (17- to 19-base-pair) DNA fragments. The mini-integrase has weak specificity for the CRISPR array. We present evidence that the Cas1 proteins form a tetramer for integration. Our findings support a model of a minimal integrase with an internal ruler mechanism that favors shorter repeats and spacers. This minimal integrase may represent the function of the ancestral Cas1 prior to Cas2 adoption.
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