ArticleBMC developmental biology2019
Interleukin-6 increases inner cell mass numbers in bovine embryos.
Article in BMC developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 72 citations in OpenAlex.
- Interleukin-6 and leukemia inhibitory factor differentially modify embryonic disk composition in post hatching bovine embryos†.Biology of reproduction · 2026Article
- Agouti-signaling protein (ASIP) improves bovine in vitro matured oocyte developmental competence and modulates lipid content.Journal of assisted reproduction and genetics · 2025Article
- The interleukin-6 signal transducer receptor subunit is required for optimal in vitro bovine embryo development†.Biology of reproduction · 2025Article
- Human Recombinant Interleukin-6 and Leukemia Inhibitory Factor Improve Inner Cell Mass Cell Number but Lack Cryoprotective Activities on In Vitro-Produced Bovine Blastocysts.Animals : an open access journal from MDPI · 2025Article
- Oviduct epithelial spheroids during in vitro culture of bovine embryos mitigate oxidative stress, improve blastocyst quality and change the embryonic transcriptome.Biological research · 2024Article
- Effect of two different commercial vaccines against bovine respiratory disease on cell-mediated immunity in Holstein cattle.Open veterinary journal · 2024Article
- Interleukin-6 supplementation improves bovine conceptus elongation and transcriptomic indicators of developmental competence†.Biology of reproduction · 2024Article
- Effects of administration of mycobacterium cell wall fraction during the periovulatory period on embryo development following superovulation in virgin dairy heifers.JDS communications · 2024Article
- Interleukin-6 Concentration in Single-Embryo Medium Is Associated with Blastocyst Formation.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Extraction of Innate Immune Genes in Dairy Cattle and the Regulation of Their Expression in Early Embryos.Genes · 2024Review
- Evaluation of the immunization of camels withOpen veterinary journal · 2024Article
- Interaction of semen with female reproductive tract tissues: what we know, what we guess and what we need to do.Animal reproduction · 2024Article
- Slow freezing cryopreservation of Korean bovine blastocysts with an additional sucrose pre-equilibration step.Frontiers in veterinary science · 2024Article
- GDF-8 improvesFrontiers in cell and developmental biology · 2024Article
- The impact of interleukin-6 (IL-6) and mesenchymal stem cell-derived IL-6 on neurological conditions.Frontiers in immunology · 2024Review
- Transcriptomic profiling of the bovine endosalpinx and endometrium to identify putative embryokines.Physiological genomics · 2023Article
- Gamma-oryzanol dose optimization in maturation or culture media forIranian journal of veterinary research · 2023Article
- Extracellular vesicles would be involved in the release and delivery of seminal TGF-β isoforms in pigs.Frontiers in veterinary science · 2023Article
- The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling.Stem cell reports · 2022Article
- Bioactive supplements influencing bovine in vitro embryo development.Journal of animal science · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWork in other species suggests that interleukin-6 (IL6) promotes early embryo development. It was unclear whether IL6 serves as an embryokine in cultured bovine embryos. This work was undertaken to elucidate the role of IL6 during in vitro bovine embryo production.
resultsTranscripts for IL6 and its two cognate receptor subunits (IL6R, IL6ST) were confirmed in bovine embryos from the 1-cell to blastocyst stages. Supplementing 100 ng/ml recombinant bovine IL6 to in vitro-produced bovine embryos at day 1, 3 or 5 increased (P < 0.05) inner cell mass (ICM) cell number and the ICM:trophectoderm (TE) ratio but not TE cell number. No increase in ICM or TE cell number was observed after supplementation of 1 or 10 ng/ml IL6 beginning at either day 1 or 5. Sequential supplementation with 100 ng/ml IL6 at both day 1 and 5 (for a total of 200 ng/ml IL6) increased (P < 0.05) ICM cell number to a greater extent than supplementing IL6 at a single time period in one study but not a second study. Additionally, providing 200 ng/ml IL6 beginning at day 1 or 5 yielded no further increase on ICM cell numbers when compared to supplementing with 100 ng/ml IL6. IL6 treatment had no effect on cleavage or blastocyst formation in group culture. However, IL6 supplementation increased cleavage and day 8 blastocyst formation when bovine embryos were cultured individually.
conclusionsThese results implicate IL6 as an embryokine that specifically increases ICM cell numbers in bovine embryos and facilitates bovine blastocyst development in embryos cultured individually.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.