Evidence map›Paper›PMID 30707509›Full record

ArticleCurrent protocols in chemical biology2019

Biochemical Stimulation of Immune Cells and Measurement of Mechanical Responses Using Atomic Force Microscopy.

Kenneth H Hu, Marc A Bruce, Jianwei Liu, Manish J Butte

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Kenneth H HuDepartment of Pathology, University of California San Francisco, San Francisco, California.
Marc A BruceMicrovolution, LLC, Sunnyvale, California.
Jianwei LiuDepartment of Chemistry, Fudan University, Shanghai, China.
Manish J ButteDepartment of Pediatrics, Division of Immunology, and Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California.

Funding

Molecular Biophysics Training Program at StanfordT32GM008294 · NIGMS · STANFORD UNIVERSITY · PI HUANG, KERWYN C. · 1989 to 2019
$7.4M
Influences of nanomechanical forces on T cellsR01GM110482 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BUTTE, MANISH J · 2014 to 2018
$1.6M
NIGMS NIH HHS R01 GM110482NIGMS NIH HHS T32 GM008294
6 · The paper itself

Abstract

This manuscript details methods to ligate cell-surface receptors on live cells with precise spatiotemporal control using an atomic force microscope (AFM) to deliver ligands. This approach can be used to image cellular responses upon activating T cell receptors when the AFM is mounted on an optical microscope. Moreover, the AFM measures forces generated by the cell during the contact. Using AFM to trigger cellular responses adds an important capability to the field of mechanobiology. We describe how to incorporate anti-CD3 antibodies or other molecules onto an AFM cantilever and how to use AFM to activate T cells. © 2019 by John Wiley & Sons, Inc.

Indexed as

AnimalsHumansMiceMicroscopy, Atomic ForceReceptors, Cell SurfaceStress, MechanicalT-LymphocytesReceptors, Cell Surfaceatomic force microscopecantilevermechanical cell forcesmechanobiologyT cell

Identifiers

PMID30707509
PMCPMC6565469

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.