ArticlePlant molecular biology2019
Tubby-like Protein 2 regulates homogalacturonan biosynthesis in Arabidopsis seed coat mucilage.
Article in Plant molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Characterization and Expression Analysis of theInternational journal of molecular sciences · 2025Article
- Identification and expression profiles of tubby‑like proteins coding genes in Brassica rapa (B. rapa) in response to hormone and drought stress.BMC plant biology · 2025Article
- The transcription factors ZAT5 and BLH2/4 regulate homogalacturonan demethylesterification in Arabidopsis seed coat mucilage.The Plant cell · 2024Article
- Molecular analyses of the tubby-like protein gene family and their response to salt and high temperature in the foxtail millet (Setaria italica).Functional & integrative genomics · 2024Article
- RNA-seq analyses on gametogenic tissues of alfalfa (Medicago sativa) revealed plant reproduction- and ploidy-related genes.BMC plant biology · 2024Article
- Overexpression of a rice Tubby-like protein-encoding gene, OsFBT4, confers tolerance to abiotic stresses.Protoplasma · 2023Article
- AtTLP2, a Tubby-like protein, plays intricate roles in abiotic stress signalling.Plant cell reports · 2023Article
- Tubby-like proteins (TLPs) transcription factor in different regulatory mechanism in plants: a review.Plant molecular biology · 2022Review
- Improvement of plant tolerance to drought stress by cotton tubby-like protein 30 through stomatal movement regulation.Journal of advanced research · 2022Article
- The Genetic Control of SEEDSTICK and LEUNIG-HOMOLOG in Seed and Fruit Development: New Insights into Cell Wall Control.Plants (Basel, Switzerland) · 2022Article
- The evolving views of the simplest pectic polysaccharides: homogalacturonan.Plant cell reports · 2022Review
- Genome-Wide Identification, Characterization, and Expression Analysis of Tubby-like Protein (TLP) Gene Family Members in Woodland Strawberry (International journal of molecular sciences · 2022Article
- Root angle is controlled byProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of theFrontiers in plant science · 2022Article
- A tubby-like protein CsTLP8 acts in the ABA signaling pathway and negatively regulates osmotic stresses tolerance during seed germination.BMC plant biology · 2021Article
- ERF4 and MYB52 transcription factors play antagonistic roles in regulating homogalacturonan de-methylesterification in Arabidopsis seed coat mucilage.The Plant cell · 2021Article
- Attracted to membranes: lipid-binding domains in plants.Plant physiology · 2021Article
- Comparative transcriptome analysis of galls from four different host plants suggests the molecular mechanism of gall development.PloS one · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
key messageA possible transcription factor TLP2 was identified to be involved in the regulation of HG biosynthesis in Arabidopsis seed mucilage. TLP2 can translocate into nucleus from plasma membrane by interacting with NF-YC3. The discovery of TLP2 gene function can further fulfill the regulatory network of pectin biosynthesis in Arabidopsis thaliana. Arabidopsis seed coat mucilage is an excellent model system to study the biosynthesis, function and regulation of pectin. Rhamnogalacturonan I (RG-I) and homogalacturonan (HG) are the major polysaccharides constituent of the Arabidopsis seed coat mucilage. Here, we identified a Tubby-like gene, Tubby-like protein 2 (TLP2), which was up-regulated in developing siliques when mucilage began to be produced. Ruthenium red (RR) staining of the seeds showed defective mucilage of tlp2-1 mutant after vigorous shaking compared to wild type (WT). Monosaccharide composition analysis revealed that the amount of total sugars and galacturonic acid (GalA) decreased significantly in the adherent mucilage (AM) of tlp2-1 mutant. Immunolabelling and dot immunoblotting analysis showed that unesterified HG decreased in the tlp2-1 mutant. Furthermore, TLP2 can translocate into nucleus by interacting with Nuclear Factor Y subunit C3 (NF-YC3) to function as a transcription factor. RNA-sequence and transactivation assays revealed that TLP2 could activate UDP-glucose 4-epimerase 1 (UGE1). In all, it is concluded that TLP2 could regulate the biosynthesis of HG possibly through the positive activation of UGE1.
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