ArticleJournal of microbiology (Seoul, Korea)2019
Gentic overexpression increases production of hypocrellin A in Shiraia bambusicola S4201.
Article in Journal of microbiology (Seoul, Korea), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Glucose-6-Phosphate Dehydrogenase ModulatesMolecules (Basel, Switzerland) · 2025Article
- Production of fungal hypocrellin photosensitizers: Exploiting bambusicolous fungi and elicitation strategies in mycelium cultures.Mycology · 2025Review
- Optimisation of hypocrellin production inMycology · 2024Article
- Biotechnological production and potential applications of hypocrellins.Applied microbiology and biotechnology · 2023Review
- Heat stress enhanced perylenequinones biosynthesis of Shiraia sp. Slf14(w) through nitric oxide formation.Applied microbiology and biotechnology · 2023Article
- Temperature-responsive regulation of the fermentation of hypocrellin A by Shiraia bambusicola (GDMCC 60438).Microbial cell factories · 2022Article
- L-Arginine enhanced perylenequinone production in the endophytic fungus Shiraia sp. Slf14(w) via NO signaling pathway.Applied microbiology and biotechnology · 2022Article
- Nitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9.Microbial cell factories · 2021Article
- Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide.Scientific reports · 2021Article
- Current State and Future Directions of Genetics and Genomics of Endophytic Fungi for Bioprospecting Efforts.Frontiers in bioengineering and biotechnology · 2021Review
- Response mechanism of hypocrellin colorants biosynthesis by Shiraia bambusicola to elicitor PB90.AMB Express · 2019Article
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Authors and funding
6 authors.
Funding
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Abstract
Hypocrellin A (HA) is a perylenequinone (PQ) isolated from Shiraia bambusicola that shows antiviral and antitumor activities, but its application is limited by the low production from wild fruiting body. A gene overexpressing method was expected to augment the production rate of HA in S. bambusicola. However, the application of this molecular biology technology in S. bambusicola was impeded by a low genetic transformation efficiency and little genomic information. To enhance the plasmid transformant ratio, the Polyethylene Glycol-mediated transformation system was established and optimized. The following green fluorescent protein (GFP) analysis showed that the gene fusion expression system we constructed with a GAPDH promoter Pgpd1 and a rapid 2A peptide was successfully expressed in the S. bambusicola S4201 strain. We successfully obtained the HA high-producing strains by overexpressing O-methyltransferase/FAD-dependent monooxygenase gene (mono) and the hydroxylase gene (hyd), which were the essential genes involved in our putative HA biosynthetic pathway. The overexpression of these two genes increased the production of HA by about 200% and 100%, respectively. In general, this study will provide a basis to identify the genes involved in the hypocrellin A biosynthesis. This improved transformation method can also be used in genetic transformation studies of other fungi.
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