ArticleJournal of microbiology (Seoul, Korea)2019
Determination of protein phosphorylation by polyacrylamide gel electrophoresis.
Article in Journal of microbiology (Seoul, Korea), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Phosphorylation of syntenin-1 by TBK1 promotes proliferation and migration of non-small cell lung cancer cells.The Journal of biological chemistry · 2025Article
- Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS-PAGE with low acrylamide concentration.FEBS open bio · 2025Article
- Structural proteomics defines a sequential priming mechanism for the progesterone receptor.Nature communications · 2025Article
- Article
- PELI2 is a negative regulator of STING signaling that is dynamically repressed during viral infection.Molecular cell · 2024Article
- Reduced liver damage and fibrosis with combined SCD Probiotics and intermittent fasting in aged rat.Journal of cellular and molecular medicine · 2024Article
- Methods optimization for the expression and purification of human calcium calmodulin-dependent protein kinase II alpha.PloS one · 2024Article
- Phosphorylation of axin within biomolecular condensates counteracts its tankyrase-mediated degradation.Journal of cell science · 2023Article
- The rejuvenating influence of young plasma on aged intestine.Journal of cellular and molecular medicine · 2023Article
- Phosphorylation of Schizosaccharomyces pombe Dss1 mediates direct binding to the ubiquitin-ligase Dma1 in vitro.Protein science : a publication of the Protein Society · 2023Article
- ER-PM Junctions on GABAergic Interneurons Are Organized by Neuregulin 2/VAP Interactions and Regulated by NMDA Receptors.International journal of molecular sciences · 2023Article
- Structural analysis and functional evaluation of the disordered ß-hexosyltransferase region fromFrontiers in bioengineering and biotechnology · 2023Article
- Level of constitutively expressed BMAL1 affects the robustness of circadian oscillations.Scientific reports · 2022Article
- Phosphoproteome Analysis Using Two-Dimensional Electrophoresis Coupled with Chemical Dephosphorylation.Foods (Basel, Switzerland) · 2022Article
- Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulators.Molecular biology of the cell · 2022Article
- Long-term mitochondrial stress induces early steps of Tau aggregation by increasing reactive oxygen species levels and affecting cellular proteostasis.Molecular biology of the cell · 2022Article
- Article
- Receptor tyrosine kinases regulate signal transduction through a liquid-liquid phase separated state.Molecular cell · 2022Article
- Subsynaptic Distribution, Lipid Raft Targeting and G Protein-Dependent Signalling of the Type 1 Cannabinoid Receptor in Synaptosomes from the Mouse Hippocampus and Frontal Cortex.Molecules (Basel, Switzerland) · 2021Article
- The inner membrane protein LapB is required for adaptation to cold stress in an LpxC-independent manner.Journal of microbiology (Seoul, Korea) · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorylation is the most important modification for protein regulation; it controls many signal transduction pathways in all organisms. While several tools to detect phosphorylated proteins have been developed to study a variety of basic cellular processes involving protein phosphorylation, these methods have several limitations. Many proteins exhibit a phosphorylation-dependent electrophoretic mobility shift (PDEMS) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the molecular mechanism responsible for this phenomenon has been elucidated recently. The method for detecting phosphorylated proteins can be simplified by the application of the PDEMS. Herein, we present a novel simple method to detect protein phosphorylation, which is based on the construction of a variant protein displaying a PDEMS. The PDEMS of proteins is caused by the distribution of negatively charged amino acids around the phosphorylation site, i.e. an electrophoretic mobility shift (EMS)-related motif (ΘX
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