Evidence map›Paper›PMID 30706307›Full record

ArticleAnnals of biomedical engineering2019

Repeated In Vitro Impact Conditioning of Astrocytes Decreases the Expression and Accumulation of Extracellular Matrix.

Addison Walker, Johntaehwan Kim, Joseph Wyatt, Abby Terlouw, Kartik Balachandran, Jeffrey Wolchok

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Article in Annals of biomedical engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Addison WalkerDepartment of Biomedical Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA.
Johntaehwan KimDepartment of Biomedical Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA.
Joseph WyattDepartment of Biomedical Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA.
Abby TerlouwDepartment of Biomedical Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA.
Kartik BalachandranDepartment of Biomedical Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA.
Jeffrey WolchokDepartment of Biomedical Engineering, University of Arkansas, 125 Engineering Hall, Fayetteville, AR, 72701, USA. jwolchok@uark.edu.ORCID http://orcid.org/0000-0003-0375-4804

Funding

National Science Foundation CMMI-1404716
6 · The paper itself

Abstract

Pathological changes to the physical and chemical properties of brain extracellular matrix (ECM) occur following injury. It is generally assumed that astrocytes play an important role in these changes. What remain unclear are the triggers that lead to changes in the regulation of ECM by astrocytes following injury. We hypothesize that mechanical stimulation triggers genotypic and phenotypic changes to astrocytes that could ultimately reshape the ECM composition of the central nervous system following injury. To explore astrocyte mechanobiology, an in vitro drop test bioreactor was employed to condition primary rat astrocytes using short duration (10 ms), high deceleration (150G) and strain (20%) impact stimuli. Experiments were designed to explore the effect of single and repeated impact (single vs. double) on mechano-sensitive behavior including cell viability; ECM gene (collagens I and IV, fibronectin, neurocan, versican) and reactivity gene [glial fibrillary acidic protein (GFAP), S100B, vimentin] expression; matrix regulatory cytokine secretion [matrix metalloproteinase 2 (MMP-2), tissue inhibitor of metalloproteinases 1 (TIMP1), transforming growth factor beta 1 (TGFβ1)]; and matrix accumulation [collagen and glycosaminoglycan (GAG)]. Experiments revealed that both ECM and reactive gliosis gene expression was significantly decreased in response to impact conditioning. The decreases for several genes, including collagen, versican, and GFAP were sensitive to impact number, suggesting mechano-sensitivity to repeated impact conditioning. The measured decreases in ECM gene expression were supported by longer-term in vitro experiments that demonstrated significant decreases in chondroitin sulfate proteoglycan (CSPG) and collagen accumulation within impact conditioned 3-D scaffolds accompanied by a 25% decrease in elastic modulus. Overall, the general trend across all samples was towards altered ECM and reactive gliosis gene expression in response to impact. These results suggest that the regulation of ECM production by astrocytes is sensitive to mechanical stimuli, and that repeated impact conditioning may increase this sensitivity.

Indexed as

BioreactorsCell Culture TechniquesGene Expression RegulationAnimalsAstrocytesCells, CulturedExtracellular MatrixExtracellular Matrix ProteinsRatsExtracellular Matrix ProteinsCentral nervous systemGlial cellsMechanobiologyNervous system

Identifiers

PMID30706307

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.