Evidence map›Paper›PMID 30705292›Full record

ArticleScientific reports2019

Characterization and immune regulation role of an immobilization antigen from Cryptocaryon irritans on groupers.

Ze-Quan Mo, Shun Xu, Donna M Cassidy-Hanley, Yan-Wei Li, Daniel Kolbin, Jennifer M Fricke, An-Xing Li, Theodore G Clark, Xue-Ming Dan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 28 citations in OpenAlex.

  1. Baohuoside I CombatedAntioxidants (Basel, Switzerland) · 2026
    Article
  2. Article
  3. Protection of Grouper AgainstFrontiers in immunology · 2022
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Ze-Quan MoJoint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Shun XuSchool of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, PR China.
Donna M Cassidy-HanleyDepartment of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Yan-Wei LiJoint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.
Daniel KolbinDepartment of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Jennifer M FrickeDepartment of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
An-Xing LiState Key Laboratory of Biocontrol/ Key Laboratory of Aquatic Product Safety (Sun Yat-Sen University), Ministry of Education, The School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, Guangdong Province, PR China.
Theodore G ClarkDepartment of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA. tgc3@cornell.edu.
Xue-Ming DanJoint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China. dxm72@scau.edu.cn.
Cornell University · USSouth China Agricultural University · CNSouth China University of Technology · CNSun Yat-sen University · CN

Funding

A Resource Center for Tetrahymena ThermophilaP40OD010964 · OD · WASHINGTON UNIVERSITY · PI DOUGLAS LEE CHALKER · 2012 to 2026
$6.5M
NIH HHS P40 OD010964
6 · The paper itself

Abstract

Immobilization antigens (i-antigens) are surface membrane proteins that are widely recognized to be the ideal candidates as vaccines antigens for immunization against Cryptocaryon irritans. In this study, we cloned a putative i-antigen gene from C. irritans, which was expressed in all three stages of the C. irritans life-cycle, and localized primarily to the cell surface. The recombinant GDCI3 i-antigen was expressed and purified using the free-living ciliate, Tetrahymena thermophila as an expression system. The purified recombinant protein was recognized by rabbit anti-C. irritans antiserum and was capable of eliciting immobilizing antibodies in rabbits and fish suggesting that the antigen itself was correctly folded. Following immunization and parasite challenge, groupers vaccinated with, recombinant GDCI3 i-antigen had a 25% cumulative percent survival rate compared to 8.3% for controls. Both non-specific and parasite-specific IgMs were generated in fish following immunization, with the levels of both increasing following challenge. Parasite-specific IgM in mucus could only be elicited after challenge of the GDCI3 i-antigen vaccinated groupers. To our knowledge, this is the first report using the Tetrahymena expression system to generate C. irritans i-antigens and investigate their use for fish vaccination.

Indexed as

AnimalsAntibodies, ProtozoanAntigens, ProtozoanBlotting, WesternCiliophoraCiliophora InfectionsFishesFluorescent Antibody TechniqueImmunoglobulin MPlasmidsProtozoan ProteinsTetrahymena thermophilaTranscriptomeAntibodies, ProtozoanAntigens, Protozoanimmobilization antigensImmunoglobulin MProtozoan Proteins

Identifiers

PMID30705292
PMCPMC6355922
OpenAlexW2912872862

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.