Evidence map›Paper›PMID 30700832›Full record

ArticleOncogene2019

Isolation and characterization of patient-derived CNS metastasis-associated stromal cell lines.

Ben Yi Tew, Christophe Legendre, Gerald C Gooden, Kyle N Johnson, Rae Anne Martinez, Jeff Kiefer, Mark Bernstein, Jennifer Glen, Loren Butry, Aleksander Hinek and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncogene, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 2 countries.

Ben Yi TewDepartment of Translational Genomics, Keck School of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA.
Christophe LegendreTranslational Genomics Research Institute, Phoenix, AZ, USA.
Gerald C GoodenDepartment of Translational Genomics, Keck School of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA.
Kyle N JohnsonTranslational Genomics Research Institute, Phoenix, AZ, USA.
Rae Anne MartinezTranslational Genomics Research Institute, Phoenix, AZ, USA.ORCID http://orcid.org/0000-0003-0262-1049
Jeff KieferTranslational Genomics Research Institute, Phoenix, AZ, USA.
Mark BernsteinDivision of Neurosurgery, University Health Network, Toronto, ON, Canada.
Jennifer GlenDivision of Neurosurgery, University Health Network, Toronto, ON, Canada.
Loren ButryDepartment of Neurosurgery, Geisinger Health System, Danville, PA, USA.
Aleksander HinekPeter Gilgan Centre for Research and Learning, Hospital for Sickkids, Toronto, ON, Canada.ORCID http://orcid.org/0000-0002-5498-6261
Steven A TomsDepartment of Neurosurgery, Lifespan Health System and Warren Alpert School of Medicine, Brown University, Providence, USA.ORCID http://orcid.org/0000-0001-8732-0522
Bodour SalhiaDepartment of Translational Genomics, Keck School of Medicine, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, USA. salhia@usc.edu.
Translational Genomics Research Institute · USUniversity of Southern California · USUniversity Health Network · CABrown University · USGeisinger Health System · USSickKids Foundation · CA

Funding

College of Medicine Phoenix Campus Vivarium Phase I ConstructionC06RR030524 · NCRR · UNIVERSITY OF ARIZONA · PI BESSELSEN, DAVID G · 2010 to 2010
$15.0M
NCRR NIH HHS C06 RR030524
6 · The paper itself

Abstract

The functional role of human derived stromal cells in the tumor microenviornment of CNS metastases (CM) remain understudied. The purpose of the current study was to isolate and characterize stromal cells of the tumor microenvironment in CM. Four different patient-derived cell lines (PDCs) of stromal and one PDC of tumorigenic origin were generated from breast or lung CM. PDCs were analyzed by DNA/RNA sequencing, DNA methylation profiling, and immunophenotypic assays. The stromal derived PDCs were termed CNS metastasis-associated stromal cells (cMASCs). Functional analysis of cMASCs was tested by co-implanting them with tumorigenic cells in mice. cMASCs displayed normal genotypes compared with tumorigenic cell lines. RNA-seq and DNA methylation analyses demonstrated that cMASCs highly resembled each other, suggesting a common cell of origin. Additionally, cMASCs revealed gene expression signatures associated with cancer associated fibroblasts (CAFs), epithelial to mesenchymal transition, mesenchymal stem cells and expressed high levels of collagen. Functionally, cMASCs restricted tumor growth, and induced desmoplasia in vivo, suggesting that cMASCs may promote a protective host response to impede tumor growth. In summary, we demonstrated the isolation, molecular characterization and functional role of human derived cMASCs, a subpopulation of cells in the microenvironment of CM that have tumor inhibitory functions.

Indexed as

AnimalsBrain NeoplasmsBreast NeoplasmsCancer-Associated FibroblastsCarcinogenesisCell Line, TumorCell ProliferationCentral Nervous SystemEpithelial-Mesenchymal TransitionFemaleHumansLung NeoplasmsMesenchymal Stem CellsMiceStromal CellsTumor Microenvironment

Identifiers

PMID30700832
PMCPMC6756000
OpenAlexW2911616931

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.