ArticleNature communications2019
Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
- Epitranscriptomic regulation of mRNA stability in pivotal transcription factors modulates the second cell fate decision.Nature communications · 2026Article
- Molecular insights into salpingitis-induced infertility and ectopic pregnancy: pathogenesis, biomarkers, and translational perspectives.Archives of gynecology and obstetrics · 2026Review
- Generation of multitissue cell-cultivated meat via multidirectional differentiation of stable porcine epiblast stem cells.Nature communications · 2026Article
- Trophectoderm-like cells from EPS cells enable generating EPS cell-derived post-implantation embryoids that complete gastrulation.Protein & cell · 2026Article
- Efficient stem cell-derived mouse embryo models for environmental studies.Developmental cell · 2026Article
- Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.The EMBO journal · 2025Article
- Stem Cell-Derived Embryo Models: Potential Platforms for Investigating Early Development and Embryotoxicity.International journal of stem cells · 2025Review
- A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation.Nature cell biology · 2025Article
- Artificial Uterus and Artificial Embryos: Unsolved Tasks.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Stem cell-based embryo models: a tool to study early human development.Science China. Life sciences · 2025Review
- Generation of transient totipotent blastomere-like stem cells by short-term high-dose Pladienolide B treatment.Science China. Life sciences · 2025Article
- The building blocks of embryo models: embryonic and extraembryonic stem cells.Cell discovery · 2025Review
- Defining core signaling pathways for supporting in vitro maintenance of pig extraembryonic endoderm (XEN) cells.Reproduction (Cambridge, England) · 2025Article
- Single-cell analysis of bidirectional reprogramming between early embryonic states identify mechanisms of differential lineage plasticities in mice.Developmental cell · 2025Article
- Reprogramming fibroblast into human iBlastoids.Nature protocols · 2024Review
- Pig blastocyst-like structure models from embryonic stem cells.Cell discovery · 2024Article
- Article
- Tissue-intrinsic beta-catenin signals antagonize Nodal-driven anterior visceral endoderm differentiation.Nature communications · 2024Article
- Blastoids generated purely from embryonic stem cells both in mice and humans.Science China. Life sciences · 2024Article
- Automated, High-Throughput Phenotypic Screening and Analysis Platform to Study Pre- and Post-Implantation Morphogenesis in Stem Cell-Derived Embryo-Like Structures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatially ordered embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic embryogenesis in vitro. However, the assembly system and developmental potential of such structures needs to be further studied. Here, we devise a nonadherent-suspension-shaking system to generate self-assembled embryo-like structures (ETX-embryoids) using mouse embryonic, trophoblast and extra-embryonic endoderm stem cells. When cultured together, the three cell types aggregate and sort into lineage-specific compartments. Signaling among these compartments results in molecular and morphogenic events that closely mimic those observed in wild-type embryos. These ETX-embryoids exhibit lumenogenesis, asymmetric patterns of gene expression for markers of mesoderm and primordial germ cell precursors, and formation of anterior visceral endoderm-like tissues. After transplantation into the pseudopregnant mouse uterus, ETX-embryoids efficiently initiate implantation and trigger the formation of decidual tissues. The ability of the three cell types to self-assemble into an embryo-like structure in vitro provides a powerful model system for studying embryogenesis.
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