Evidence map›Paper›PMID 30700652›Full record

ArticleThe Journal of veterinary medical science2019

Rapid and sensitive detection of elephant endotheliotropic herpesvirus 1 (EEHV1) in blood by loop-mediated isothermal amplification (LAMP).

Kazuya Takehana, Teruo Kinjyo, Manabu Nemoto, Keita Matsuno

Open access · diamondAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 4 institutions in 1 country.

Kazuya TakehanaIchihara Elephant Kingdom Zoological Park, Ichihara, Chiba 290-0521, Japan.
Teruo KinjyoOkinawa Zoo and Museum, Okinawa-shi, Okinawa 904-0021, Japan.
Manabu NemotoEquine Research Institute, Japan Racing Association, Shimotsuke, Tochigi 329-0412, Japan.
Keita MatsunoLaboratory of Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
Hokkaido University · JPJapan Racing Association · JPOkinawa Prefectural Museum · JPTokyo Zoological Park Society · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Elephant endotheliotropic herpesvirus type 1 (EEHV1) is the most important causative agent of an acute fatal hemorrhagic disease in Asian elephants (Elephas maximus). We employed loop-mediated isothermal amplification (LAMP) to develop a rapid and simple detection method for EEHV1 in blood. When used to test 21 clinical samples collected in Japan, the EEHV1 assay correctly identified one positive and 20 negative clinical samples. It was observed that when samples were spiked with synthetic DNA plasmids including EEHV1 polymerase gene, the detection limit of the LAMP assay was 10

Indexed as

AnimalsDNA-Directed DNA PolymeraseDNA PrimersElephantsFemaleHerpesviridaeHerpesviridae InfectionsMaleNucleic Acid Amplification TechniquesPolymerase Chain ReactionSensitivity and SpecificityTime FactorsDNA-Directed DNA PolymeraseDNA Primersacute hemorrhagic diseaseAsian elephants (Elephas maximus)elephant endotheliotropic herpesvirus (EEHV)loop-mediated isothermal amplification (LAMP)polymerase chain reaction (PCR)

Identifiers

PMID30700652
PMCPMC6451909
OpenAlexW2914092178

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.