Evidence map›Paper›PMID 30700268›Full record

ArticleBMC genomics2019

Usability of reference-free transcriptome assemblies for detection of differential expression: a case study on Aethionema arabicum dimorphic seeds.

Per K I Wilhelmsson, Jake O Chandler, Noe Fernandez-Pozo, Kai Graeber, Kristian K Ullrich, Waheed Arshad, Safina Khan, Johannes A Hofberger, Karl Buchta, Patrick P Edger and 4 more

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Article
  3. Frontiers in plant science · 2024
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  7. Review
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  10. Review
  11. Article
  12. Article
  13. Genomic Blocks inFrontiers in plant science · 2020
    Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 5 countries.

Per K I WilhelmssonPlant Cell Biology, Faculty of Biology, University of Marburg, 35043, Marburg, Germany.ORCID https://orcid.org/0000-0002-8578-3387
Jake O ChandlerSchool of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK.ORCID https://orcid.org/0000-0003-0955-9241
Noe Fernandez-PozoPlant Cell Biology, Faculty of Biology, University of Marburg, 35043, Marburg, Germany.
Kai GraeberSchool of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK.
Kristian K UllrichPlant Cell Biology, Faculty of Biology, University of Marburg, 35043, Marburg, Germany.
Waheed ArshadSchool of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK.ORCID https://orcid.org/0000-0002-9413-2279
Safina KhanSchool of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK.
Johannes A HofbergerBiosystematics Group, Wageningen University, Wageningen, 6708 PB, The Netherlands.
Karl BuchtaPlant Cell Biology, Faculty of Biology, University of Marburg, 35043, Marburg, Germany.
Patrick P EdgerDepartment of Horticulture, Michigan State University, East Lansing, MI, 48864, USA.
J Chris PiresDivision of Biological Sciences, University of Missouri, Columbia, MO, 65211, USA.
M Eric SchranzBiosystematics Group, Wageningen University, Wageningen, 6708 PB, The Netherlands.
Gerhard Leubner-MetzgerSchool of Biological Sciences, Royal Holloway University of London, Egham, Surrey, TW20 0EX, UK. gerhard.leubner@rhul.ac.uk.ORCID https://orcid.org/0000-0002-6045-8713
Stefan A RensingPlant Cell Biology, Faculty of Biology, University of Marburg, 35043, Marburg, Germany. stefan.rensing@biologie.uni-marburg.de.ORCID http://orcid.org/0000-0002-0225-873X
Royal Holloway University of London · GBPhilipps University of Marburg · DEWageningen University & Research · NLMichigan State University · USUniversity of Freiburg · DEUniversity of Missouri · US

Funding

Biotechnology and Biological Sciences Research Council BB/M00192X/1 BB/M000583/1Deutsche Forschungsgemeinschaft RE 1697/8-1Natural Environment Research Council NE/L002485/1Netherlands Organization for International Cooperation in Higher Education 849.13.004
6 · The paper itself

Abstract

backgroundRNA-sequencing analysis is increasingly utilized to study gene expression in non-model organisms without sequenced genomes. Aethionema arabicum (Brassicaceae) exhibits seed dimorphism as a bet-hedging strategy - producing both a less dormant mucilaginous (M

resultsA de novo transcriptome assembly was generated using sequences from M

conclusionsWhilst there was only modest overlap of DEGs identified in reference-free versus -dependent approaches, the resulting GO analysis was concordant in both approaches. The identified differences in dry seed transcriptomes suggest mechanisms underpinning previously identified contrasts between morphology and germination behaviour of M

Indexed as

Gene Expression Regulation, PlantTranscriptomeBrassicaceaeGene Expression ProfilingGene OntologyGenome, PlantGerminationHigh-Throughput Nucleotide SequencingMolecular Sequence AnnotationPlant ProteinsSeedsPlant ProteinsAethionema arabicumDimorphic seedsReference and reference-freeRNA-seqTranscriptome

Identifiers

PMID30700268
PMCPMC6354389
OpenAlexW2913454006

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.