ArticleThe Annals of otology, rhinology, and laryngology2019
Mouse Model of Tracheal Replacement With Electrospun Nanofiber Scaffolds.
Article in The Annals of otology, rhinology, and laryngology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Applications of Electrospinning for Tissue Engineering in Otolaryngology.The Annals of otology, rhinology, and laryngology · 2021Pooled it
- Characteristics of 3D-Printed Polycaprolactone Tracheal Scaffolds Implanted In Vivo.Tissue engineering and regenerative medicine · 2025Article
- Assessing the Biocompatibility and Regeneration of Electrospun-Nanofiber Composite Tracheal Grafts.The Laryngoscope · 2024Article
- Long-Term Chondrocyte Retention in Partially Decellularized Tracheal Grafts.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2024Article
- Optimization of Chondrocyte Viability in Partially Decellularized Tracheal Grafts.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2023Article
- Current Status and Future Outlook of Additive Manufacturing Technologies for the Reconstruction of the Trachea.Journal of functional biomaterials · 2023Review
- A Multimodal Approach to Quantify Chondrocyte Viability for Airway Tissue Engineering.The Laryngoscope · 2023Article
- Tracheal Macrophages During Regeneration and Repair of Long-Segment Airway Defects.The Laryngoscope · 2022Article
- The Growing Medical Need for Tracheal Replacement: Reconstructive Strategies Should Overcome Their Limits.Frontiers in bioengineering and biotechnology · 2022Review
- Modulation of Synthetic Tracheal Grafts with Extracellular Matrix Coatings.Bioengineering (Basel, Switzerland) · 2021Article
- Standardization of Microcomputed Tomography for Tracheal Tissue Engineering Analysis.Tissue engineering. Part C, Methods · 2020Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Deconstructing tissue engineered trachea: Assessing the role of synthetic scaffolds, segmental replacement and cell seeding on graft performance.Acta biomaterialia · 2020Article
- Regeneration of partially decellularized tracheal scaffolds in a mouse model of orthotopic tracheal replacement.Journal of tissue engineeringArticle
- Tissue-engineered composite tracheal grafts create mechanically stable and biocompatible airway replacements.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
objectivesThe clinical experience with tissue-engineered tracheal grafts (TETGs) has been fraught with graft stenosis and delayed epithelialization. A mouse model of orthotopic replacement that recapitulates the clinical findings would facilitate the study of the cellular and molecular mechanisms underlying graft stenosis.
methodsElectrospun nanofiber tracheal scaffolds were created using nonresorbable (polyethylene terephthalate + polyurethane) and co-electrospun resorbable (polylactide-co-caprolactone/polyglycolic acid) polymers (n = 10/group). Biomechanical testing was performed to compare load displacement of nanofiber scaffolds to native mouse tracheas. Mice underwent orthotopic tracheal replacement with syngeneic grafts (n = 5) and nonresorbable (n = 10) and resorbable (n = 10) scaffolds. Tissue at the anastomosis was evaluated using hematoxylin and eosin (H&E), K5+ basal cells were evaluated with the help of immunofluorescence testing, and cellular infiltration of the scaffold was quantified. Micro computed tomography was performed to assess graft patency and correlate radiographic and histologic findings with respiratory symptoms.
resultsSynthetic scaffolds were supraphysiologic in compression tests compared to native mouse trachea ( P < .0001). Nonresorbable scaffolds were stiffer than resorbable scaffolds ( P = .0004). Eighty percent of syngeneic recipients survived to the study endpoint of 60 days postoperatively. Mean survival with nonresorbable scaffolds was 11.40 ± 7.31 days and 6.70 ± 3.95 days with resorbable scaffolds ( P = .095). Stenosis manifested with tissue overgrowth in nonresorbable scaffolds and malacia in resorbable scaffolds. Quantification of scaffold cellular infiltration correlated with length of survival in resorbable scaffolds (R
conclusionGraft stenosis seen in orthotopic tracheal replacement with synthetic tracheal scaffolds can be modeled in mice. The wide array of lineage tracing and transgenic mouse models available will permit future investigation of the cellular and molecular mechanisms underlying TETG stenosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.