ArticleACS chemical biology2019
Detecting Secretory Proteins by Acoustic Droplet Ejection in Multiplexed High-Throughput Applications.
Article in ACS chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Proteomics applications in next generation induced pluripotent stem cell models.Expert review of proteomics · 2024Review
- Paracatalytic induction: Subverting specificity in hedgehog protein autoprocessing with small molecules.Methods in enzymology · 2023Article
- High-Throughput Cellular Thermal Shift Assays in Research and Drug Discovery.SLAS discovery : advancing life sciences R & D · 2020Article
- Ipomoeassin F Binds Sec61α to Inhibit Protein Translocation.Journal of the American Chemical Society · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
Nearly one-third of the encoded proteome is comprised of secretory proteins that enable communication between cells and organ systems, playing a ubiquitous role in human health and disease. High-throughput detection of secreted proteins would enhance efforts to identify therapies for secretion-related diseases. Using the Z mutant of alpha-1 antitrypsin as a human secretory model, we have developed 1536-well high-throughput screening assays that utilize acoustic droplet ejection to transfer nanoliter volumes of sample for protein quantification. Among them, the acoustic reverse phase protein array (acoustic RPPA) is a multiplexable, low-cost immunodetection technology for native, endogenously secreted proteins from physiologically relevant model systems like stem cells that is compatible with plate-based instrumentation. Parallel assay profiling with the LOPAC
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Registered trials
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