ArticleLab on a chip2019
A microfluidic platform for the characterisation of membrane active antimicrobials.
Article in Lab on a chip, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
- The Ion Permeability of DNA Nanotube Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Uniform Microscale Thermoresponsive Liposomes for Controlled Release Above Body Temperature.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Systematic investigation of double emulsion dewetting dynamics for the robust production of giant unilamellar vesicles.Lab on a chip · 2026Article
- Designing AI-generated antimicrobials for targeting bacterial microdomains.Scientific reports · 2025Article
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- A microfluidic platform for extraction and analysis of bacterial genomic DNA.Lab on a chip · 2025Article
- Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs.Advanced healthcare materials · 2024Review
- Elastin-like polypeptide coacervates as reversibly triggerable compartments for synthetic cells.Communications chemistry · 2024Article
- Glass Microdroplet Generator for Lipid-Based Double Emulsion Production.Micromachines · 2024Article
- Membrane-Active Peptides and Their Potential Biomedical Application.Pharmaceutics · 2023Review
- Article
- Designing Biological Microsensors with Chiral Nematic Liquid Crystal Droplets.ACS applied materials & interfaces · 2022Article
- Measuring Thousands of Single-Vesicle Leakage Events Reveals the Mode of Action of Antimicrobial Peptides.Analytical chemistry · 2022Article
- A Multiplexed Cell-Free Assay to Screen for Antimicrobial Peptides in Double Emulsion Droplets.Angewandte Chemie (International ed. in English) · 2022Article
- An ultrasensitive microfluidic approach reveals correlations between the physico-chemical and biological activity of experimental peptide antibiotics.Scientific reports · 2022Article
- A Microfluidic Platform for Sequential Assembly and Separation of Synthetic Cell Models.ACS synthetic biology · 2021Article
- Article
- Standardizing characterization of membrane active peptides with microfluidics.Biomicrofluidics · 2021Article
- Switching Cytolytic Nanopores into Antimicrobial Fractal Ruptures by a Single Side Chain Mutation.ACS nano · 2021Article
- To Close or to Collapse: The Role of Charges on Membrane Stability upon Pore Formation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
The spread of bacterial resistance against conventional antibiotics generates a great need for the discovery of novel antimicrobials. Polypeptide antibiotics constitute a promising class of antimicrobial agents that favour attack on bacterial membranes. However, efficient measurement platforms for evaluating their mechanisms of action in a systematic manner are lacking. Here we report an integrated lab-on-a-chip multilayer microfluidic platform to quantify the membranolytic efficacy of such antibiotics. The platform is a biomimetic vesicle-based screening assay, which generates giant unilamellar vesicles (GUVs) in physiologically relevant buffers on demand. Hundreds of these GUVs are individually immobilised downstream in physical traps connected to separate perfusion inlets that facilitate controlled antibiotic delivery. Antibiotic efficacy is expressed as a function of the time needed for an encapsulated dye to leak out of the GUVs as a result of antibiotic treatment. This proof-of-principle study probes the dose response of an archetypal polypeptide antibiotic cecropin B on GUVs mimicking bacterial membranes. The results of the study provide a foundation for engineering quantitative, high-throughput microfluidics devices for screening antibiotics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.