ArticleAmerican journal of clinical and experimental urology2018
Development of a murine intravesical orthotopic human bladder cancer (mio-hBC) model.
Article in American journal of clinical and experimental urology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 9 citations in OpenAlex.
- Optical imaging guidance in oncologic surgery and interventional oncology.Pharmacological research · 2025Review
- A novel approach to engineering three-dimensional bladder tumor models for drug testing.Scientific reports · 2024Article
- Pharmacological Inhibition of Endogenous Hydrogen Sulfide Production Slows Bladder Cancer Progression in an Intravesical Murine Model.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Development of high-efficiency superparamagnetic drug delivery system with MPI imaging capability.Frontiers in bioengineering and biotechnology · 2024Article
- Review
- Evaluation of direct intramural injection to the bladder wall as a method for developing orthotopic tumor models.Animal models and experimental medicine · 2022Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a murine intravesical orthotopic human bladder cancer (mio-hBC) model for the establishment of superficial urothelial cell carcinomas. In this model we catheterize female atyhmic nude mice and pre-treat the bladder with poly-L-lysine for 15 minutes, followed by intravesical instillation of luciferase-transfected human UM-UC-3 cells. Cancer cells are quantified by bioluminescent imaging which has been validated by small animal ultrasound. Poly-L-lysine pre-treatment increased engraftment rate (84.4%) and resulted in faster growing tumors than trypsin pre-treatment. In addition, tumors respond through a decrease in growth and increase in apoptosis to chemotherapy with mitomycin C. Previous intravesical models utilized KU7 cells which have been later determined to be of non-bladder origin. They display markers consistent with HeLa cells, requiring a need for a true intravesical bladder model. Efficient engraftment and rapid superficial growth patterning of the human bladder tumor differentiate this in vivo orthotopic model from previous bladder models.
Indexed as
Identifiers
30697580PMC6334200W2989648459What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.