ArticleJournal of animal science and biotechnology2019
Protein profiles of enzymatically isolated rumen epithelium in sheep fed a fibrous diet.
Article in Journal of animal science and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Quantitative differences in rumen epithelium proteins and detection of lysine acetylation in lambs fed a low or high metabolizable energy diet.Journal of animal science · 2025Article
- Multi-omics revealed the mechanism of feed efficiency in sheep by the combined action of the host and rumen microbiota.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- 3D sheep rumen epithelial structures driven from single cells in vitro.Veterinary research · 2023Article
- Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne.Proteomes · 2023Article
- Single-cell transcriptomic landscape of the sheep rumen provides insights into physiological programming development and adaptation of digestive strategies.Zoological research · 2022Article
- Characterization of Accessible Chromatin Regions in Cattle Rumen Epithelial Tissue during Weaning.Genes · 2022Article
- Cross-tissue single-cell transcriptomic landscape reveals the key cell subtypes and their potential roles in the nutrient absorption and metabolism in dairy cattle.Journal of advanced research · 2022Article
- Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype.PloS one · 2022Article
- Diet Transition from High-Forage to High-Concentrate Alters Rumen Bacterial Community Composition, Epithelial Transcriptomes and Ruminal Fermentation Parameters in Dairy Cows.Animals : an open access journal from MDPI · 2021Article
- Brisket Disease Is Associated with Lower Volatile Fatty Acid Production and Altered Rumen Microbiome in Holstein Heifers.Animals : an open access journal from MDPI · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe rumen wall plays a major role in efficient transfer of digested nutrients in the rumen to peripheral tissues through the portal venous system. Some of these substrates are metabolised in the epithelium during this process. To identify the specific proteins involved in these processes, we used proteomic technologies. Protein extracts were prepared from ventral rumen tissue of six sheep fed a fibrous diet at 1.5× maintenance energy requirements. Using a newly developed method, we were able to enzymatically isolate the epithelial cells from underlying tissue layers, thus allowing cytosol and membrane fractions to be independently analysed using liquid chromatography tandem mass spectrometry (LC MS/MS).
resultsUsing our procedure we identified 570 epithelial proteins in the
conclusionOur research has developed a procedure to isolate rumen epithelium proteins from the underlying tissue layers so that they may be profiled using proteomic technologies. The approach improves the number of proteins that can be profiled that are specific to the epithelium of the rumen wall. It provides new insights into the proteins of structural and nutritional importance in the rumen epithelium, that carry out nutrient transport and metabolism, cell growth and signalling.
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