Molecular Cloning, Expression and Characterization of Para Flagellar Rod Protein 1 of

Biswa Ranjan Maharana, Anup Kumar Tewari, Naduvanahalli Rajanna Sudhakar, Chinmoy Mishra

Abstract read

In one paragraph

Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

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1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Evaluation of the Immunoprotective Potential of Recombinant Paraflagellar Rod Proteins ofVaccines · 2020
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Biswa Ranjan MaharanaDept. of Parasitology, Referral Veterinary Diagnostic & Extension Center, LUVAS, Haryana, India.

Anup Kumar TewariDivision of Parasitology, Indian Veterinary Research Institute, Izatnagar, U.P., India.

Naduvanahalli Rajanna SudhakarDivision of Parasitology, Indian Veterinary Research Institute, Izatnagar, U.P., India.

Chinmoy MishraDivision of Animal Genetics and Breeding, OUAT, Odisha, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAntigenic variation allows the trypanosomes to evade the potentially destructive host immune response and is an important reason for failure to develop a protective vaccine. Among the non-variant structural proteins, paraflagellar rod protein (PFR) is a prospective vaccine target owing to its role in the active movement of the parasite.

methodsThe PFR1 gene was cloned in pET-32a expression vector and after confirmation by restriction digestion, expressed as a Histidine-tagged fusion protein, in BL21 DE3 strain of

resultsThe results of sequencing, restriction digestion analysis, and PCR reaction revealed that cloning of PFR1 gene in pET-32a expression vector and the results of SDS PAGE and Western blot further confirmed its homogeneity and purity. The

conclusionWe report here the molecular cloning, heterologous expression, and characterization of PFR1, a constituent protein of PFR. Due to its conserved nature, the PFR1 protein could be a prospective vaccine target against multiple

Indexed as

Para flagellar rod protein 1SDS-PAGETrypanosoma evansiWestern blot

Identifiers

PMID30697316

PMCPMC6348229

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.