ArticleScientific reports2019
Nanoparticle administration method in cell culture alters particle-cell interaction.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 98 citations in OpenAlex.
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- Gold Nanoraspberries for Surface-Enhanced Raman Scattering: Synthesis, Optimization, and Characterization.ACS omega · 2025Article
- The effects of photochemical aging and interactions with secondary organic aerosols on cellular toxicity of combustion particles.Journal of aerosol science · 2025Article
- The impact of cell density variations on nanoparticle uptake across bioprinted A549 gradients.Frontiers in bioengineering and biotechnology · 2025Article
- Nanotherapeutic Heterogeneity: Sources, Effects, and Solutions.Small (Weinheim an der Bergstrasse, Germany) · 2024Review
- Nanoparticle accumulation and penetration in 3D tumor models: the effect of size, shape, and surface charge.Frontiers in cell and developmental biology · 2024Article
- Nanoparticles-induced potential toxicity on human health: Applications, toxicity mechanisms, and evaluation models.MedComm · 2023Review
- The Functions of Cholera Toxin Subunit B as a Modulator of Silica Nanoparticle Endocytosis.Toxins · 2023Article
- Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy.Nature communications · 2023Article
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- An In Vitro Dosimetry Tool for the Numerical Transport Modeling of Engineered Nanomaterials Powered by the Enalos RiskGONE Cloud Platform.Nanomaterials (Basel, Switzerland) · 2022Article
- Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes.Journal of nanobiotechnology · 2022Article
- Substrate stiffness reduces particle uptake by epithelial cells and macrophages in a size-dependent manner through mechanoregulation.Nanoscale · 2022Article
- Microenvironmental Behaviour of Nanotheranostic Systems for Controlled Oxidative Stress and Cancer Treatment.Nanomaterials (Basel, Switzerland) · 2022Review
- Impairing proliferation of glioblastoma multiforme with CD44+ selective conjugated polymer nanoparticles.Scientific reports · 2022Article
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- A predictive microfluidic model of human glioblastoma to assess trafficking of blood-brain barrier-penetrant nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- "Cells-to-cDNA on Chip": Phenotypic Assessment and Gene Expression Analysis from Live Cells in Nanoliter Volumes Using Droplet Microarrays.Advanced healthcare materials · 2022Article
- Increasing the Efficacy of Gold Nanorod Uptake in Stem Cell-Derived Therapeutic Cells: Implications for Stem Cell Labeling and Optical Coherence Tomography Imaging.ACS applied nano materials · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a highly interdisciplinary field, working with nanoparticles in a biomedical context requires a robust understanding of soft matter physics, colloidal behaviors, nano-characterization methods, biology, and bio-nano interactions. When reporting results, it can be easy to overlook simple, seemingly trivial experimental details. In this context, we set out to understand how in vitro technique, specifically the way we administer particles in 2D culture, can influence experimental outcomes. Gold nanoparticles coated with poly(vinylpyrrolidone) were added to J774A.1 mouse monocyte/macrophage cultures as either a concentrated bolus, a bolus then mixed via aspiration, or pre-mixed in cell culture media. Particle-cell interaction was monitored via inductively coupled plasma-optical emission spectroscopy and we found that particles administered in a concentrated dose interacted more with cells compared to the pre-mixed administration method. Spectroscopy studies reveal that the initial formation of the protein corona upon introduction to cell culture media may be responsible for the differences in particle-cell interaction. Modeling of particle deposition using the in vitro sedimentation, diffusion and dosimetry model helped to clarify what particle phenomena may be occurring at the cellular interface. We found that particle administration method in vitro has an effect on particle-cell interactions (i.e. cellular adsorption and uptake). Initial introduction of particles in to complex biological media has a lasting effect on the formation of the protein corona, which in turn mediates particle-cell interaction. It is of note that a minor detail, the way in which we administer particles in cell culture, can have a significant effect on what we observe regarding particle interactions in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.