ArticleScientific reports2019
Caspase-4 disaggregates lipopolysaccharide micelles via LPS-CARD interaction.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- When folding CARDs wins the game: molecular snapshots of lipopolysaccharide sensing by the non-canonical inflammasome.Biochemical Society transactions · 2026Review
- Structural insights into ligand-induced CARD assembly in inflammatory caspases.Research square · 2026Article
- LPS-induced structural reorganization and polymerization drive noncanonical inflammasome activation.Science advances · 2026Article
- Article
- Beyond the Toll-Like Receptor 4. Structure-Dependent Lipopolysaccharide Recognition Systems: How far are we?ChemMedChem · 2025Review
- Recognition ofJournal of oral microbiology · 2025Article
- The Secretion of Inflammatory Cytokines Triggered by TLR2 Through Calcium-Dependent and Calcium-Independent Pathways in Keratinocytes.Mediators of inflammation · 2024Article
- Regulation of alveolar macrophage death in pulmonary fibrosis: a review.Apoptosis : an international journal on programmed cell death · 2023Review
- Caspase-mediated LPS sensing and pyroptosis signaling inScience advances · 2023Article
- Microbiota-Derived Extracellular Vesicles Detected in Human Blood from Healthy Donors.International journal of molecular sciences · 2022Article
- Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome.Nature immunology · 2022Article
- A Fluorescence-Polarization-Based Lipopolysaccharide-Caspase-4 Interaction Assay for the Development of Inhibitors.Molecules (Basel, Switzerland) · 2022Article
- The role of caspase-1, caspase-4 and NLRP3 in regulating the host cell response evoked by uropathogenic Escherichia coli.Scientific reports · 2022Article
- ClearColi as a platform for untagged pneumococcal surface protein A production: cultivation strategy, bioreactor culture, and purification.Applied microbiology and biotechnology · 2022Article
- Pyroptosis and inflammasomes in diabetic wound healing.Frontiers in endocrinology · 2022Review
- Human Renal Fibroblasts, but Not Renal Epithelial Cells, Induce IL-1β Release during a UropathogenicCells · 2021Article
- Human guanylate binding proteins: nanomachines orchestrating host defense.The FEBS journal · 2021Review
- Galectin-3 promotes noncanonical inflammasome activation through intracellular binding to lipopolysaccharide glycans.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Pulmonary Inflammation and KRAS Mutation in Lung Cancer.Advances in experimental medicine and biology · 2021Article
- Direct binding of polymeric GBP1 to LPS disrupts bacterial cell envelope functions.The EMBO journal · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipopolysaccharides (LPS) are a major component of the outer membrane of Gram-negative bacteria and are pathogen-associated molecular patterns recognized by the TLR4/MD2 complex that induces an inflammatory response. Recently, the cytosolic receptors caspase-4/-5/-11 that bind LPS inside the cell and trigger inflammasome activation or pyroptosis, have been identified. Despite the important roles of caspase-4 in human immune responses, few studies have investigated its biochemical characteristics and interactions with LPS. Since caspase-4 (C258A) purified from an Escherichia coli host forms aggregates, monomeric proteins including full-length caspase-4, caspase-4 (C258A), and the CARD domain of caspase-4 have been purified from the insect cell system. Here, we report the overexpression and purification of monomeric caspase-4 (C258A) and CARD domain from E. coli and demonstrate that purified caspase-4 (C258A) and CARD domain bind large LPS micelles and disaggregate them to small complexes. As the molar ratio of caspase-4 to LPS increases, the size of the caspase-4/LPS complex decreases. Our results present a new function of caspase-4 and set the stage for structural and biochemical studies, and drug discovery targeting LPS/caspase-4 interactions by establishing a facile purification method to obtain large quantities of purified caspase-4 (C258A) and the CARD domain.
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