Evidence map›Paper›PMID 30696841›Full record

ArticleScientific reports2019

Rapid diagnosis of Mycoplasma pneumonia infection by denaturation bubble-mediated strand exchange amplification: comparison with LAMP and real-time PCR.

Wenqiang Shi, Manman Wei, Qing Wang, Hongwei Wang, Cuiping Ma, Chao Shi

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Epidemiological investigation of porcineFrontiers in veterinary science · 2025
    Article
  3. Article
  4. Article
  5. Ultrasensitive, Specific, and Rapid Detection ofFrontiers in microbiology · 2022
    Article
  6. Article
  7. Article
  8. Detection of canine parvovirus and feline panleukopenia virus in fecal samples by strand exchange amplification.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Wenqiang ShiCollege of Life Sciences, Qingdao University, Qingdao, 266071, PR China.
Manman WeiShandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Qing WangThe Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao, 266101, PR China.
Hongwei WangThe Clinical Laboratory Department of the Affiliated Hospital of Qingdao University, Qingdao, 266101, PR China.
Cuiping MaShandong Provincial Key Laboratory of Biochemical Engineering, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Chao ShiCollege of Life Sciences, Qingdao University, Qingdao, 266071, PR China. sc169@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

M. pneumoniae infection is often ignored due to its similar clinical symptom with respiratory tract infections caused by bacteria or viruses, and thus leading to misdiagnosis and delayed treatment. It is critical to develop a rapid, sensitive and specific diagnosis method. Denaturation Bubble-mediated Strand Exchange Amplification (SEA) was established, which is an isothermal method with only a primer pair and one Bst DNA polymerase. Notably, colorimetric SEA assay was developed with simple visual readout, making instrument-independent in detection step. The method could detect as low as 1.0 × 10

Indexed as

Nucleic Acid Amplification TechniquesHumansMolecular Diagnostic TechniquesMycoplasma pneumoniaePneumonia, MycoplasmaReal-Time Polymerase Chain ReactionReproducibility of ResultsRNA, Ribosomal, 16SSensitivity and SpecificityRNA, Ribosomal, 16S

Identifiers

PMID30696841
PMCPMC6351683

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.