ArticleMolecular biology reports2019
Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Mulberry (Morus alba L.) under different abiotic stresses.
Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 60 citations in OpenAlex.
- Identification and Validation of Reference Genes for Quantitative Real-Time PCR inPlants (Basel, Switzerland) · 2025Article
- Characterization of two reductasesFrontiers in plant science · 2025Article
- Cross-species stability of reference genes in medicinal plants Arnica montana and Arnica chamissonis.Scientific reports · 2024Article
- Integrated transcriptomic and metabolomic analyses elucidate the mechanism of flavonoid biosynthesis in the regulation of mulberry seed germination under salt stress.BMC plant biology · 2024Article
- Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns ofInternational journal of molecular sciences · 2023Article
- Diversity of fungal pathogens in leaf spot disease of Indian mulberry and its management.Heliyon · 2023Article
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- Chromosome constitution and genetic relationships of Morus spp. revealed by genomic in situ hybridization.BMC plant biology · 2023Article
- Transcriptome Analysis Reveals Genes Associated with Flooding Tolerance in Mulberry Plants.Life (Basel, Switzerland) · 2023Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of Nitraria sibirica under various abiotic stresses.BMC plant biology · 2022Article
- Comprehensive analysis of the MYB transcription factor gene family in Morus alba.BMC plant biology · 2022Article
- Functional Characterization of Flavanone 3-Hydroxylase (F3H) and Its Role in Anthocyanin and Flavonoid Biosynthesis in Mulberry.Molecules (Basel, Switzerland) · 2022Article
- Identification of suitable reference genes for quantitative reverse transcription PCR inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
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- Selection of reference genes for quantitative real-time PCR normalization in European quail tissues.Molecular biology reports · 2021Article
- Genome-wide analysis of the lignin toolbox forPeerJ · 2021Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Plants (Basel, Switzerland) · 2020Article
- Selection of suitable reference genes for qRT-PCR expression analysis of Codonopsis pilosula under different experimental conditions.Molecular biology reports · 2020Article
- Indirect regeneration and genetic fidelity analysis of acclimated plantlets through SCoT and ISSR markers inBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- The Selection of Reference Genes for Quantitative Real-Time PCR in the Ashidan Yak Mammary Gland During Lactation and Dry Period.Animals : an open access journal from MDPI · 2019Article
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7 authors at 2 institutions in 1 country.
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Abstract
Mulberry (Morus alba L.) is the sole food source for the mulberry silkworm, Bombyx mori and therefore important for sericulture industry. Different abiotic stress conditions like drought, salt, heat and cold stress adversely affect the productivity and quality of mulberry leaves. Quantitative real time PCR (qPCR) is a reliable and widely used method to identify abiotic stress responsive genes and molecular mechanism in different plant species. Selection of suitable reference genes is important requirement for normalizing the expression of genes through qRT-PCR study. In the present study, we have selected eight candidate reference genes in mulberry for analyzing their expression stability in different abiotic stress treatments including drought, salt, heat and cold stresses. The expression stability of these reference genes was determined using geNorm, NormFinder and RefFinder statistical algorithms. The results showed that Ubiquitin and protein phosphatase 2A regulatory subunit A (PP2A) were the most stable genes across all the treatment samples. However, analysis of individual stresses revealed different expression profiles and stability of reference genes. Actin3 and PP2A were most stable in drought and salt conditions respectively. RPL3 most preferred in heat stress and Ubiquitin was most stable in cold stress. We propose the ubiquitin and PP2A are the preferred reference genes for normalization of gene expression data from abiotic stresses. In addition, Actin3 are preferred for drought stress, PP2A for salt stress, RPL3 for heat stress and Ubiquitin for cold stress studies.
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