Evidence map›Paper›PMID 30693156›Full record

ArticleApplications in plant sciences2019

A high-throughput method of analyzing multiple plant defensive compounds in minimized sample mass.

Chandra N Jack, Shawna L Rowe, Stephanie S Porter, Maren L Friesen

Open access · goldAbstract read
In one paragraph

Article in Applications in plant sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Enhanced HSP70 binding to mBMC plant biology · 2024
    Article
  4. Plant signaling & behavior · 2024
    Article
  5. Article
  6. Rapid evolution ofEcology and evolution · 2019
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Chandra N JackDepartment of Plant Pathology Washington State University Pullman Washington 99164 USA.ORCID 0000-0003-0167-1271
Shawna L RoweDepartment of Plant Biology Michigan State University East Lansing Michigan 48824 USA.
Stephanie S PorterSchool of Biological Sciences Washington State University Vancouver Washington 98686 USA.
Maren L FriesenDepartment of Plant Pathology Washington State University Pullman Washington 99164 USA.
Washington State University · USMichigan State University · USWashington State University Vancouver · US

Funding

Plant Biotechnology for Health and SustainabilityT32GM110523 · NIGMS · MICHIGAN STATE UNIVERSITY · PI LAST, ROBERT LOUIS · 2014 to 2023
$2.2M
NIGMS NIH HHS T32 GM110523
6 · The paper itself

Abstract

premise of the studyCurrent methods for quantifying herbivore-induced alterations in plant biochemistry are often unusable by researchers due to practical constraints. We present a cost-effective, high-throughput protocol to quantify multiple biochemical responses from small plant tissue samples using spectrophotometric techniques. METHODS AND

resultsUsing

conclusionsCurrent protocols can require 500 mg of tissue, but our assays detect activity in less than 10 mg. Our protocol takes two people 6 h to run any of the assays on 300 samples in triplicate, or all of the assays on 20 samples. Our protocol enables researchers to plan complex experiments that compare local versus systemic plant responses, quantify environmental and genetic variation, and measure population-level variation.

Indexed as

microplateperoxidaseplant defense responsepolyphenol oxidaseprotease inhibitorsprotein quantification

Identifiers

PMID30693156
PMCPMC6342235
OpenAlexW2910169826

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.