Evidence map›Paper›PMID 30692706›Full record

ArticleActa informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH2018

Isolation, Characterization and Proliferation of Cancer Cells from Breast Cancer Patients.

Wahyu Widowati, Yusuf Heriady, Dian Ratih Laksmitawati, Diana Krisanti Jasaputra, Teresa Liliana Wargasetia, Rizal Rizal, Fajar Sukma Perdana, Annisa Amalia, Annisa Arlisyah, Zakiyatul Khoiriyah and 2 more

Abstract read
In one paragraph

Article in Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. From Donor to the Lab: A Fascinating Journey of Primary Cell Lines.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 1 country.

Wahyu WidowatiMedical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Yusuf HeriadyFaculty of Medicine, Bandung Islamic University, Bandung, Indonesia.
Dian Ratih LaksmitawatiFaculty of Pharmacy, Pancasila University, Jakarta, Indonesia.
Diana Krisanti JasaputraMedical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Teresa Liliana WargasetiaMedical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Rizal RizalMedical Research Centre, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Fajar Sukma PerdanaBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia.
Annisa AmaliaBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia.
Annisa ArlisyahBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia.
Zakiyatul KhoiriyahBiomolecular and Biomedical Research Centre, Aretha Medika Utama, Bandung, West Java, Indonesia.
Ahmad FariedDepartment of Neurosurgery and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia.
Mawar SubangkitLaboratory of Veterinary Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
Maranatha Christian University · IDBandung Islamic University · IDIPB University · IDPadjadjaran University · IDPancasila University · ID

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe most cases of cancer death, which are in the first rank among cancers suffered by women is breast cancer. The breast cancer therapy for patients has been done, but still not optimal, so it is necessary to understand the mechanism of therapy in model cell of breast cancer.

aimThis study aim to develop an isolation technique of breast cancer cell from patients as a cancer cell model. MATERIAL AND

methodsBreast cancer cell isolation is performed by enzymatic methods using the collagen I and hyaluronidase. Then, breast cancer cells were characterized using flow cytometry based on the CD44/CD24 expression where MDA-MB468 and MCF-7 breast cancer cell lines were used as positive controls. Estrogen receptor (ER), progesterone receptor (PR), p53, HER2, and Ki67 expression were assessed using an immunohistochemistry assay. RESULT AND DISCUSSION: The morphology of cancer cells was fibroblast like cells on the day 7

conclusionThese cells belonged to a basal type of breast carcinoma and expressed CD44

Indexed as

breast cancerCD24 antigenCD44 antigencell linesenzymatic assay

Identifiers

PMID30692706
PMCPMC6311122
OpenAlexW2904835884

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.