ArticleBiological psychiatry. Cognitive neuroscience and neuroimaging2019
Awake Mouse Imaging: From Two-Photon Microscopy to Blood Oxygen Level-Dependent Functional Magnetic Resonance Imaging.
Article in Biological psychiatry. Cognitive neuroscience and neuroimaging, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Where do we stand on fMRI in awake mice?Cerebral cortex (New York, N.Y. : 1991) · 2024Pooled it
- Simultaneous single-cell calcium imaging of neuronal population activity and brain-wide BOLD fMRI.Nature methods · 2026Article
- Brain-Wide Activations Related to Forepaw Force Control Identified by Behaving Mouse FMRI.eNeuro · 2026Article
- Voxel-accurate MRI-microscopy Correlation Enables AI-powered Prediction of Brain Disease States.Theranostics · 2026Article
- Arteries and veins in awake mice using two-photon microscopy.Journal of anatomy · 2025Article
- Carbonic anhydrase inhibitors prevent presymptomatic capillary flow disturbances in a model of cerebral amyloidosis.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025Article
- Neurovascular coupling: a review of spontaneous neocortical dynamics linking neuronal activity to hemodynamics and what we have learned from the rodent brain.Journal of neurophysiology · 2025Review
- High-resolution awake mouse fMRI at 14 tesla.eLife · 2025Article
- Low-cost physiology and behavioral monitor for intravital imaging in small mammals.Neurophotonics · 2025Article
- A non-invasive approach to awake mouse fMRI compatible with multi-modal techniques.Imaging neuroscience (Cambridge, Mass.) · 2025Article
- High-resolution awake mouse fMRI at 14 Tesla.bioRxiv : the preprint server for biology · 2024Article
- Novel inductively coupled ear-bars (ICEs) to enhance restored fMRI signal from susceptibility compensation in rats.Cerebral cortex (New York, N.Y. : 1991) · 2024Article
- NBS-SNI, an extension of the network-based statistic: Abnormal functional connections between important structural actors.Network neuroscience (Cambridge, Mass.) · 2024Article
- FDA-approved carbonic anhydrase inhibitors reduce amyloid β pathology and improve cognition, by ameliorating cerebrovascular health and glial fitness.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2023Article
- Dynamic 3D imaging of cerebral blood flow in awake mice using self-supervised-learning-enhanced optical coherence Doppler tomography.Communications biology · 2023Article
- A quantitative model for human neurovascular coupling with translated mechanisms from animals.PLoS computational biology · 2023Article
- Contribution of Intravital Neuroimaging to Study Animal Models of Multiple Sclerosis.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2023Review
- Shortwave infrared fluorescence imaging of peripheral organs in awake and freely moving mice.Frontiers in neuroscience · 2023Article
- Generation of a whole-brain hemodynamic response function and sex-specific differences in cerebral processing of mechano-sensation in mice detected by BOLD fMRI.Frontiers in neuroscience · 2023Article
- Do sparse brain activity patterns underlie human cognition?NeuroImage · 2022Article
Corrections and comments
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Authors and funding
24 authors at 5 institutions in 1 country.
Funding
Abstract
backgroundFunctional magnetic resonance imaging (fMRI) in awake behaving mice is well positioned to bridge the detailed cellular-level view of brain activity, which has become available owing to recent advances in microscopic optical imaging and genetics, to the macroscopic scale of human noninvasive observables. However, though microscopic (e.g., two-photon imaging) studies in behaving mice have become a reality in many laboratories, awake mouse fMRI remains a challenge. Owing to variability in behavior among animals, performing all types of measurements within the same subject is highly desirable and can lead to higher scientific rigor.
methodsWe demonstrated blood oxygenation level-dependent fMRI in awake mice implanted with long-term cranial windows that allowed optical access for microscopic imaging modalities and optogenetic stimulation. We started with two-photon imaging of single-vessel diameter changes (n = 1). Next, we implemented intrinsic optical imaging of blood oxygenation and flow combined with laser speckle imaging of blood flow obtaining a mesoscopic picture of the hemodynamic response (n = 16). Then we obtained corresponding blood oxygenation level-dependent fMRI data (n = 5). All measurements could be performed in the same mice in response to identical sensory and optogenetic stimuli.
resultsThe cranial window did not deteriorate the quality of fMRI and allowed alternation between imaging modalities in each subject.
conclusionsThis report provides a proof of feasibility for multiscale imaging approaches in awake mice. In the future, this protocol could be extended to include complex cognitive behaviors translatable to humans, such as sensory discrimination or attention.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.