ArticleJournal of structural biology2019
Consideration of sample motion in cryo-tomography based on alignment residual interpolation.
Article in Journal of structural biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- A ternary complex of MIPs in the A-tubule of basal bodies and axonemes depends on RIB22 and the EF-hand domain of RIB72A inMolecular biology of the cell · 2025Article
- The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.bioRxiv : the preprint server for biology · 2024Article
- Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.The Journal of cell biology · 2024Article
- Poc1 is a basal body inner junction protein that promotes triplet microtubule integrity and interconnections.bioRxiv : the preprint server for biology · 2023Article
- Computational methods forFrontiers in cellular and infection microbiology · 2023Review
- ScipionTomo: Towards cryo-electron tomography software integration, reproducibility, and validation.Journal of structural biology · 2022Article
- Electron cryo-tomography structure of axonemal doublet microtubule fromLife science alliance · 2022Article
- AreTomo: An integrated software package for automated marker-free, motion-corrected cryo-electron tomographic alignment and reconstruction.Journal of structural biology: X · 2022Article
- Article
- Proteomic analysis of microtubule inner proteins (MIPs) in Rib72 nullMolecular biology of the cell · 2021Article
- Structure and dynamics of photoreceptor sensory cilia.Pflugers Archiv : European journal of physiology · 2021Review
- Step-by-step guide to efficient subtomogram averaging of virus-like particles with Dynamo.PLoS biology · 2021Article
- Current data processing strategies for cryo-electron tomography and subtomogram averaging.The Biochemical journal · 2021Review
- Robust and ultrafast fiducial marker correspondence in electron tomography by a two-stage algorithm considering local constraints.Bioinformatics (Oxford, England) · 2021Article
- Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å in cells.Nature methods · 2021Article
- Arrangements of proteins at reconstituted synaptic vesicle fusion sites depend on membrane separation.FEBS letters · 2020Article
- Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM.Nature communications · 2020Article
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3 authors.
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Abstract
Recently, it has been shown that the resolution in cryo-tomography could be improved by considering the sample motion in tilt-series alignment and reconstruction, where a set of quadratic polynomials were used to model this motion. One requirement of this polynomial method is the optimization of a large number of parameters, which may limit its practical applicability. In this work, we propose an alternative method for modeling the sample motion. Starting from the standard fiducial-based tilt-series alignment, the method uses the alignment residual as local estimates of the sample motion at the 3D fiducial positions. Then, a scattered data interpolation technique characterized by its smoothness and a closed-form solution is applied to model the sample motion. The motion model is then integrated in the tomographic reconstruction. The new method improves the tomogram quality similar to the polynomial one, with the important advantage that the determination of the motion model is greatly simplified, thereby overcoming one of the major limitations of the polynomial model. Therefore, the new method is expected to make the beam-induced motion correction methodology more accessible to the cryoET community.
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