ArticleThe Journal of antimicrobial chemotherapy2019
ChloS-HRM, a novel assay to identify chloramphenicol-susceptible Escherichia coli and Klebsiella pneumoniae in Malawi.
Article in The Journal of antimicrobial chemotherapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 24 citations in OpenAlex.
- Prevalence and Differential Plasmid Versus Chromosomal Distribution of Ribosome-Targeting Antibiotic Resistance Genes inAntibiotics (Basel, Switzerland) · 2026Article
- Gut Microbial Composition and Antibiotic Resistance Profiles in Dairy Calves with Diarrhea.Life (Basel, Switzerland) · 2024Article
- Molecular mechanisms of re-emerging chloramphenicol susceptibility in extended-spectrum beta-lactamase-producing Enterobacterales.Nature communications · 2024Article
- Private and well drinking water are reservoirs for antimicrobial resistant bacteria.npj antimicrobials and resistance · 2024Article
- Development and proof-of-concept demonstration of a clinical metagenomics method for the rapid detection of bloodstream infection.BMC medical genomics · 2024Article
- Regulation Transcriptional of Antibiotic Resistance Genes (ARGs) in Bacteria Isolated from WWTP.Current microbiology · 2023Article
- Evaluation of the Ability to Form Biofilms in KPC-Producing and ESBL-ProducingAntibiotics (Basel, Switzerland) · 2023Article
- Molecular surveillance reveals widespread colonisation by carbapenemase and extended spectrum beta-lactamase producing organisms in neonatal units in Kenya and Nigeria.Antimicrobial resistance and infection control · 2023Article
- Prevalence and Characterization ofInfection and drug resistance · 2023Article
- Amoxicillin and thiamphenicol treatments may influence the co-selection of resistance genes in the chicken gut microbiota.Scientific reports · 2022Article
- Piperacillin/tazobactam-resistant, cephalosporin-susceptibleMicrobial genomics · 2022Article
- A novel air-dried multiplex high-resolution melt assay for the detection of extended-spectrum β-lactamase and carbapenemase genes.Journal of global antimicrobial resistance · 2021Article
- Correlation between antibiotic resistance and phylogenetic types among multidrug-resistantIranian journal of basic medical sciences · 2021Article
- Amino AcidBiology · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
objectivesChloramphenicol is a broad-spectrum antimicrobial widely available in sub-Saharan Africa. With susceptibility re-emerging among Enterobacteriaceae in Blantyre, Malawi, we designed and evaluated a new high-resolution melt (HRM) RT-PCR assay, ChloS-HRM, to identify chloramphenicol-susceptible infections in a hospital setting.
methodsSeventy-two previously whole-genome sequenced isolates of Escherichia coli and Klebsiella pneumoniae from the Queen Elizabeth Central Hospital, Malawi, were subjected to determination of chloramphenicol MICs. Primers were designed to detect 18 chloramphenicol resistance genes that produce seven distinct peaks correlating with different gene groups (catA1, catA2, catA3, catB2, catB group 3, cmlA and floR) following HRM analysis. ChloS-HRM results were compared with MIC and WGS results.
resultsChloS-HRM correctly identified 15 of 17 phenotypically susceptible isolates and 54 of 55 resistant isolates, giving an accuracy of 88% in identifying susceptibility and 98% in identifying resistance. WGS identified 16 of 17 susceptible and 54 of 55 resistant isolates, giving an accuracy of 94% in identifying susceptibility and 98% in identifying resistance. The single false-susceptible result had no detectable gene by ChloS-HRM or WGS. Compared with WGS, ChloS-HRM had 100% sensitivity and specificity for catA (catA1-3), cmlA and floR, and 96% specificity for catB; sensitivity could not be estimated due to the lack of catB in the clinical sample collection. The overall agreement between MIC and HRM was 96% and between MIC and WGS it was 97%.
conclusionsChloS-HRM could support antimicrobial stewardship in enabling de-escalation from third-generation cephalosporins by identifying chloramphenicol-susceptible infections. This would be valuable in areas with chloramphenicol-susceptible MDR and XDR Enterobacteriaceae.
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