ArticleInternational journal of epidemiology2019
Proof of concept for quantitative urine NMR metabolomics pipeline for large-scale epidemiology and genetics.
Article in International journal of epidemiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Longitudinal urine metabolomics predicts high-grade brain injury in very preterm infants.Pediatric research · 2026Article
- Exploring metabolic signatures in urine using NMR for improved prognosis of gliomas.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Nuclear magnetic resonance combined with genetic algorithm with linear discriminant analysis (GA-LDA) is a suitable model for discriminating urinary metabolomic profiles of individuals with glycemic disorders.Annals of medicine · 2025Article
- Influence of pre-analytical factors and biological variability on the urinary volatilome.Analytical and bioanalytical chemistry · 2025Article
- Securing the Future of NMR Metabolomics Reproducibility: A Call for Standardized Reporting.Analytical chemistry · 2025Review
- Exploring the Impact of Urinary Metal Exposure on Blood Lipid Profiles in Chinese Adults: Mediating Roles of Health-Related Indicators.Journal of the American Heart Association · 2025Article
- Nuclear Magnetic Resonance Based Metabolomics-A Rising Star in Traditional Chinese Medicine Research.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Diagnosis of deep vein thrombosis from γ-butylbetaine and L-carnitine in plasma and urine based on untargeted and targeted metabolomics.Scientific reports · 2025Article
- Urinary metabolomics provide insights into coronary artery disease in individuals with type 1 diabetes.Cardiovascular diabetology · 2024Article
- Basic accuracy of a 1D NOESY with presaturation method using standard solutions of amino and maleic acids.Analytical and bioanalytical chemistry · 2024Article
- Clinical and biochemical associations of urinary metabolites: quantitative epidemiological approach on renal-cardiometabolic biomarkers.International journal of epidemiology · 2024Article
- Approach to the diagnosis and management of dysbiosis.Frontiers in nutrition · 2024Review
- A metabolic readout of the urine metabolome of COVID-19 patients.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- NMR Metabolomics Methods for Investigating Disease.Analytical chemistry · 2023Review
- Quantitative NMR Methods in Metabolomics.Handbook of experimental pharmacology · 2023Article
- There is always glucose in normal urine: unspecific excretion associated with serum glucose and glomerular filtration rate.International journal of epidemiology · 2022Article
- Cluster Analysis Statistical Spectroscopy for the Identification of Metabolites inMetabolites · 2022Article
- Characteristics of Normalization Methods in Quantitative Urinary Metabolomics-Implications for Epidemiological Applications and Interpretations.Biomolecules · 2022Article
- A Real-Life Reproducibility Assessment for NMR Metabolomics.Diagnostics (Basel, Switzerland) · 2022Article
- Cohort Profile: 46 years of follow-up of the Northern Finland Birth Cohort 1966 (NFBC1966).International journal of epidemiology · 2022Article
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18 authors.
Funding
Abstract
backgroundQuantitative molecular data from urine are rare in epidemiology and genetics. NMR spectroscopy could provide these data in high throughput, and it has already been applied in epidemiological settings to analyse urine samples. However, quantitative protocols for large-scale applications are not available.
methodsWe describe in detail how to prepare urine samples and perform NMR experiments to obtain quantitative metabolic information. Semi-automated quantitative line shape fitting analyses were set up for 43 metabolites and applied to data from various analytical test samples and from 1004 individuals from a population-based epidemiological cohort. Novel analyses on how urine metabolites associate with quantitative serum NMR metabolomics data (61 metabolic measures; n = 995) were performed. In addition, confirmatory genome-wide analyses of urine metabolites were conducted (n = 578). The fully automated quantitative regression-based spectral analysis is demonstrated for creatinine and glucose (n = 4548).
resultsIntra-assay metabolite variations were mostly <5%, indicating high robustness and accuracy of urine NMR spectroscopy methodology per se. Intra-individual metabolite variations were large, ranging from 6% to 194%. However, population-based inter-individual metabolite variations were even larger (from 14% to 1655%), providing a sound base for epidemiological applications. Metabolic associations between urine and serum were found to be clearly weaker than those within serum and within urine, indicating that urinary metabolomics data provide independent metabolic information. Two previous genome-wide hits for formate and 2-hydroxyisobutyrate were replicated at genome-wide significance.
conclusionQuantitative urine metabolomics data suggest broad novelty for systems epidemiology. A roadmap for an open access methodology is provided.
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