Evidence map›Paper›PMID 30689096›Full record

ArticleMolecular genetics and genomics : MGG2019

Agrobacterium-mediated Tnt1 mutagenesis of moss protonemal filaments and generation of stable mutants with impaired gametophyte.

Boominathan Mohanasundaram, Vyankatesh B Rajmane, Sukanya V Jogdand, Amey J Bhide, Anjan K Banerjee

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular genetics and genomics : MGG, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. The MossThe Plant cell · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Boominathan MohanasundaramIndian Institute of Science Education and Research (IISER, Pune), Dr. Homi Bhabha Road, Pune, Maharashtra, 411 008, India.
Vyankatesh B RajmaneIndian Institute of Science Education and Research (IISER, Pune), Dr. Homi Bhabha Road, Pune, Maharashtra, 411 008, India.
Sukanya V JogdandIndian Institute of Science Education and Research (IISER, Pune), Dr. Homi Bhabha Road, Pune, Maharashtra, 411 008, India.
Amey J BhideIndian Institute of Science Education and Research (IISER, Pune), Dr. Homi Bhabha Road, Pune, Maharashtra, 411 008, India.
Anjan K BanerjeeIndian Institute of Science Education and Research (IISER, Pune), Dr. Homi Bhabha Road, Pune, Maharashtra, 411 008, India. akb@iiserpune.ac.in.ORCID http://orcid.org/0000-0002-1825-7926
Indian Institute of Science Education and Research Pune · IN

Funding

Department of Science and Technology, Govt of India EMR/2016/004852
6 · The paper itself

Abstract

The gametophyte of moss exhibits a simple body plan, yet its growth is regulated by complex developmental phenomena similar to angiosperms. Because moss can be easily maintained under laboratory conditions, amenable for gene targeting and the availability of genome sequence, P. patens has become an attractive model system for studying evolutionary traits. Until date, there has been no Agrobacterium-mediated Tnt1 mutagenesis protocol for haploid protonemal filaments of moss. Hence, we attempted to use the intact tobacco Tnt1 retrotransposon as a mutagen for P. patens. Bioinformatic analysis of initiator methionyl-tRNA (Met-tRNA

Indexed as

Mutagenesis, InsertionalAgrobacteriumBase SequenceBryopsidaChromosomes, PlantDNA, PlantGenome, PlantGerm Cells, PlantNicotianaPhylogenyPlants, Genetically ModifiedRetroelementsRNA, Transfer, MetSequence Homology, Nucleic AcidTransformation, GeneticDNA, PlantMet-tRNA(i)(Met)RetroelementsRNA, Transfer, MetForward genetic screenGametophyte developmentInitiator methionyl-tRNALTRMossPhyscomitrella patensTnt1 retrotransposon

Identifiers

PMID30689096
OpenAlexW2911358030

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.