Evidence map›Paper›PMID 30689044›Full record

ArticleEuropean spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society2019

GEORG SCHMORL PRIZE OF THE GERMAN SPINE SOCIETY (DWG) 2018: combined inflammatory and mechanical stress weakens the annulus fibrosus: evidences from a loaded bovine AF organ culture.

Taryn Saggese, Graciosa Q Teixeira, Kelly Wade, Lydia Moll, Anita Ignatius, Hans-Joachim Wilke, Raquel M Goncalves, Cornelia Neidlinger-Wilke

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In one paragraph

Article in European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

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  13. Immuno-stimulatory capacity of decorin in the rat tail intervertebral disc and the mechanical consequence of resultant inflammation.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Taryn SaggeseInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.
Graciosa Q TeixeiraInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.ORCID http://orcid.org/0000-0003-4206-6326
Kelly WadeInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.
Lydia MollInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.
Anita IgnatiusInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.
Hans-Joachim WilkeInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.
Raquel M GoncalvesInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany.
Cornelia Neidlinger-WilkeInstitute of Orthopaedic Research and Biomechanics, Trauma Research Centre, Ulm University, Helmholtzstraße 14, 89081, Ulm, Germany. cornelia.neidlinger-wilke@uni-ulm.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe pathomechanism of annulus fibrosus (AF) failure is still unknown. We hypothesise that mechanical overload and an inflammatory microenvironment contribute to AF structural weakening. Therefore, the objective of this study was to investigate the influence of these factors on the AF, particularly the translamellar bridging network (TLBN) which connects the AF lamellae.

methodsA bovine AF organ culture (AF-OC) model of standardised AF rings was used to study the individual and combined effects of cyclic tensile strain (CTS) and IL-1β (1 ng/mL) culture medium supplementation. AF-OCs were analysed for PGE

resultsThe combination of CTS + IL-1β led to a significant increase in PGE

conclusionOur findings suggest that CTS and IL-1β act synergistically to increase pro-inflammatory and catabolic molecules within the AF, particularly the TLBN, leading to a weakening of the tissue. This standardised model enables the investigation of AF/TLBN structure-function relationship and is a platform to test AF-focused therapeutics. These slides can be retrieved under Electronic Supplementary Material.

Indexed as

Stress, MechanicalAnimalsAnnulus FibrosusCattleCell SurvivalCyclooxygenase 2DinoprostoneFibrillinsImmunohistochemistryInterleukin-1betaInterleukin-6Matrix Metalloproteinase 3MicroscopyModels, AnimalCyclooxygenase 2DinoprostoneFibrillinsInterleukin-1betaInterleukin-6Matrix Metalloproteinase 3Annulus fibrosusDisc herniationInflammationMechanical loadingOrgan culture

Identifiers

PMID30689044

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.