ArticleeLife2019
FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- An FGF21-based fusion protein ameliorates mitochondrial dysfunction and promotes recovery post spinal cord injury.Materials today. Bio · 2026Article
- Live-cell 3D-SIM of Rift Valley fever virus NSs filaments reveals a polygon web architecture.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms.Nature methods · 2026Article
- Structural glycobiology - from enzymes to organelles.Biochemical Society transactions · 2025Review
- Advancing Nanomedicine Through Electron Microscopy: Insights Into Nanoparticle Cellular Interactions and Biomedical Applications.International journal of nanomedicine · 2025Review
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- Engineering poly- and micelleplexes for nucleic acid delivery - A reflection on their endosomal escape.Journal of controlled release : official journal of the Controlled Release Society · 2023Review
- CryomicroscopyFaraday discussions · 2022Article
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- Covalent and non-covalent coupling of a AuNanoscale advances · 2021Article
- Facile Synthesis of Peptide-Conjugated Gold Nanoclusters with Different Lengths.Nanomaterials (Basel, Switzerland) · 2021Article
- Gold nanoparticles and tilt pairs to assess protein flexibility by cryo-electron microscopy.Ultramicroscopy · 2021Article
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Nanoscopy for endosomal escape quantification.Nanoscale advances · 2021Review
- Correlated cryogenic fluorescence microscopy and electron cryo-tomography shows that exogenous TRIM5α can form hexagonal lattices or autophagy aggregates in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies.Journal of micromechanics and microengineering : structures, devices, and systems · 2019Article
- A method for structure prediction of metal-ligand interfaces of hybrid nanoparticles.Nature communications · 2019Article
- Brief update on endocytosis of nanomedicines.Advanced drug delivery reviews · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The fibroblast growth factor FGF21 was labeled with molecularly defined gold nanoparticles (AuNPs), applied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET). Most AuNPs were in pairs about 80 Å apart, on the outer cell surface. Pairs of AuNPs were also abundant inside the cells in clathrin-coated vesicles and endosomes. AuNPs were present but no longer paired in multivesicular bodies. FGF21 could thus be tracked along the endocytotic pathway. The methods developed here to visualize signaling coupled to endocytosis can be applied to a wide variety of cargo and may be extended to studies of other intracellular transactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.