ArticleInsect science2020
Ecdysone receptor isoforms play distinct roles in larval-pupal-adult transition in Leptinotarsa decemlineata.
Article in Insect science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- HcEcR Regulates Chitin Biosynthesis via 20E Signaling Rather Than Direct Transcriptional Activation in Hyphantria cunea Larvae.Archives of insect biochemistry and physiology · 2026Article
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- Molecular Characterization and Expression of the Ecdysone Receptor and Ultraspiracle Genes in the Wheat Blossom Midge,Insects · 2025Article
- Nano-delivery platform with strong protection and efficient delivery: preparation of self-assembled RNA pesticide with dual RNAi targets against Apolygus lucorum.Journal of nanobiotechnology · 2025Article
- 20-Hydroxyecdysone Mediates Reproductive Diapause inInternational journal of molecular sciences · 2024Article
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- Functional Analysis of a Multiple-Domain CTL15 in the Innate Immunity, Eclosion, and Reproduction ofCells · 2023Article
- Sodium Benzoate Delays the Development ofFrontiers in microbiology · 2022Article
- The uncommon function and mechanism of the common enzyme glyceraldehyde-3-phosphate dehydrogenase in the metamorphosis ofFrontiers in bioengineering and biotechnology · 2022Article
- Combined Transcriptomic Analysis and RNA Interference Reveal the Effects of Methoxyfenozide on Ecdysone Signaling Pathway ofInternational journal of molecular sciences · 2021Article
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- Post-transcriptional regulation of insect metamorphosis and oogenesis.Cellular and molecular life sciences : CMLS · 2020Review
- Two Splice Isoforms ofFrontiers in physiology · 2020Article
- Importance of Taiman in Larval-Pupal Transition inFrontiers in physiology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
A heterodimer of two nuclear receptors, ecdysone receptor (EcR) and ultraspiracle, mediates 20-hydroxyecdysone (20E) signaling to modulate many aspects in insect life, such as molting and metamorphosis, reproduction, diapause and innate immunity. In the present paper, we intended to determine the isoform-specific roles of EcR during larval-pupal-adult transition in the Colorado potato beetle. Double-stranded RNAs (dsRNAs) were prepared using the common (dsEcR) or isoform-specific (dsEcRA, dsEcRB1) regions of EcR as templates. Ingestion of either dsEcR or dsEcRA, rather than dsEcRB1, by the penultimate (3rd) and final (4th) instar larvae caused failure of larval-pupal and pupal-adult ecdysis. The RNA interference (RNAi) larvae remained as prepupae, or became deformed pupae and adults. Determination of messenger RNA (mRNA) levels of EcR isoforms found that LdEcRA regulates the expression of LdEcRB1. Moreover, silencing the two EcR transcripts, LdEcRA or LdEcRB1 reduced the mRNA levels of Ldspo and Ldsad, and lowered 20E titer. In contrast, the expression levels of HR3, HR4, E74 and E75 were significantly decreased in the LdEcR or LdEcRA RNAi larvae, but not in LdEcRB1 depleted specimens. Dietary supplement with 20E did not restore the expression of five 20E signaling genes (USP, HR3, HR4, E74 and E75), and only partially alleviated the pupation defects in dsEcR- or dsEcRA-fed beetles. These data suggest that EcR plays isoform-specific roles in the regulation of ecdysteroidogenesis and the transduction of 20E signal in L. decemlineata.
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Registered trials
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