ArticleBiophysical journal2019
Probe Sensitivity to Cortical versus Intracellular Cytoskeletal Network Stiffness.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
35 citing papers in PubMed, 60 citations in OpenAlex.
- Review
- Global alignment and local curvature of microtubules in mouse fibroblasts are robust against perturbations of vimentin and actin.Soft matter · 2025Article
- Cancer cell stiffening via CoQNature communications · 2024Article
- Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation.Journal of the Royal Society, Interface · 2024Article
- Vimentin is a key regulator of cell mechanosensing through opposite actions on actomyosin and microtubule networks.Communications biology · 2024Article
- Analyzing force measurements of multi-cellular clusters comprising indeterminate geometries.Biomechanics and modeling in mechanobiology · 2024Article
- Quantifying both viscoelasticity and surface tension: Why sharp tips overestimate cell stiffness.Biophysical journal · 2024Article
- Glucocorticoid-Induced Ocular Hypertension and Glaucoma.Clinical ophthalmology (Auckland, N.Z.) · 2024Review
- Vimentin regulates nuclear segmentation in neutrophils.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Environmental stiffness restores mechanical homeostasis in vimentin-depleted cells.Scientific reports · 2023Article
- Article
- Cells in the polyaneuploid cancer cell (PACC) state have increased metastatic potential.Clinical & experimental metastasis · 2023Article
- Nuclear lamin isoforms differentially contribute to LINC complex-dependent nucleocytoskeletal coupling and whole-cell mechanics.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Roles of vimentin in health and disease.Genes & development · 2022Review
- Vimentin intermediate filaments and filamentous actin form unexpected interpenetrating networks that redefine the cell cortex.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- How does plasticity of migration help tumor cells to avoid treatment: Cytoskeletal regulators and potential markers.Frontiers in pharmacology · 2022Review
- Cell senescence alters responses of porcine trabecular meshwork cells to shear stress.Frontiers in cell and developmental biology · 2022Article
- ALD-R491 regulates vimentin filament stability and solubility, cell contractile force, cell migration speed and directionality.Frontiers in cell and developmental biology · 2022Article
- Expression of vimentin alters cell mechanics, cell-cell adhesion, and gene expression profiles suggesting the induction of a hybrid EMT in human mammary epithelial cells.Frontiers in cell and developmental biology · 2022Article
- Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy.Nature biomedical engineering · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
In development, wound healing, and pathology, cell biomechanical properties are increasingly recognized as being of central importance. To measure these properties, experimental probes of various types have been developed, but how each probe reflects the properties of heterogeneous cell regions has remained obscure. To better understand differences attributable to the probe technology, as well as to define the relative sensitivity of each probe to different cellular structures, here we took a comprehensive approach. We studied two cell types-Schlemm's canal endothelial cells and mouse embryonic fibroblasts (MEFs)-using four different probe technologies: 1) atomic force microscopy (AFM) with sharp tip, 2) AFM with round tip, 3) optical magnetic twisting cytometry (OMTC), and 4) traction microscopy (TM). Perturbation of Schlemm's canal cells with dexamethasone treatment, α-actinin overexpression, or RhoA overexpression caused increases in traction reported by TM and stiffness reported by sharp-tip AFM as compared to corresponding controls. By contrast, under these same experimental conditions, stiffness reported by round-tip AFM and by OMTC indicated little change. Knockout (KO) of vimentin in MEFs caused a diminution of traction reported by TM, as well as stiffness reported by sharp-tip and round-tip AFM. However, stiffness reported by OMTC in vimentin-KO MEFs was greater than in wild type. Finite-element analysis demonstrated that this paradoxical OMTC result in vimentin-KO MEFs could be attributed to reduced cell thickness. Our results also suggest that vimentin contributes not only to intracellular network stiffness but also cortex stiffness. Taken together, this evidence suggests that AFM sharp tip and TM emphasize properties of the actin-rich shell of the cell, whereas round-tip AFM and OMTC emphasize those of the noncortical intracellular network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.