ArticleJournal of biophotonics2019
Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.
Article in Journal of biophotonics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Third harmonic generation microscopy reveals structure and mucus dynamics in human airway epithelium models.iScience · 2026Article
- Short-pulsed AI-augmented third harmonic generation imaging visualizes negative-contrast cell nuclei in fresh mouse liver tissue without optical-induced damage.Biomedical optics express · 2026Article
- Effects of Molecular Aggregation on Dynamic Third-Order Nonlinear Optical Responses: Oligo(thiophene-benzothiadiazole) as a Case Study.Journal of chemical theory and computation · 2026Article
- Optical super-resolution histology of formalin-fixed paraffin-embedded tissue samples: challenges and opportunities.Nature communications · 2025Review
- Article
- Multi-Observer Study on Diagnostic Accuracy of Pediatric Renal Tumors Imaged with Higher-Harmonic-Generation Microscopy.Cancers · 2025Article
- Article
- Ultrasound-guided photoacoustic (US-PA) tomography of the breast: Biochemical differentiation using intrinsic tissue markers-lipids, collagen and hemoglobin with histopathologic correlation.Scientific reports · 2024Article
- Keratin from Animal By-Products: Structure, Characterization, Extraction and Application-A Review.Polymers · 2024Review
- Characterization of lamina propria remodeling in pediatric eosinophilic esophagitis using second harmonic generation microscopy.Translational medicine communications · 2024Article
- Effect of tissue fixation on the optical properties of structural components assessed by non-linear microscopy imaging.Biomedical optics express · 2023Article
- Rapid On-Site Pathology Visualization of COVID-19 Characteristics Using Higher Harmonic Generation Microscopy.American journal of respiratory and critical care medicine · 2023Article
- Two-Photon Endoscopy: State of the Art and Perspectives.Molecular imaging and biology · 2023Review
- Leukocyte differentiation in bronchoalveolar lavage fluids using higher harmonic generation microscopy and deep learning.PloS one · 2023Article
- Beyond the snapshot: optimizing prognostication and prediction by moving from fixed to functional multidimensional cancer pathology.The Journal of pathology · 2022Review
- Article
- Cellular Plasticity during Metastasis: New Insights Provided by Intravital Microscopy.Cold Spring Harbor perspectives in medicine · 2020Review
- Compact portable multiphoton microscopy reveals histopathological hallmarks of unprocessed lung tumor tissue in real time.Translational biophotonics · 2020Article
- Simultaneous label-free autofluorescence-multiharmonic microscopy and beyond.APL photonics · 2019Article
- Second and third harmonic generation microscopy visualizes key structural components in fresh unprocessed healthy human breast tissue.Journal of biophotonics · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Real-time assessment of excised tissue may help to improve surgical results in breast tumor surgeries. Here, as a step towards this purpose, the potential of second and third harmonic generation (SHG, THG) microscopy is explored. SHG and THG are nonlinear optical microscopic techniques that do not require labeling of tissue to generate 3D images with intrinsic depth-sectioning at sub-cellular resolution. Until now, this technique had been applied on fixated breast tissue or to visualize the stroma only, whereas most tumors start in the lobules and ducts. Here, SHG/THG images of freshly excised unprocessed healthy human tissue are shown to reveal key breast components-lobules, ducts, fat tissue, connective tissue and blood vessels, in good agreement with hematoxylin and eosin histology. DNA staining of fresh unprocessed mouse breast tissue was performed to aid in the identification of cell nuclei in label-free THG images. Furthermore, 2- and 3-photon excited auto-fluorescence images of mouse and human tissue are collected for comparison. The SHG/THG imaging modalities generate high quality images of freshly excised tissue in less than a minute with an information content comparable to that of the gold standard, histopathology. Therefore, SHG/THG microscopy is a promising tool for real-time assessment of excised tissue during surgery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.