ArticlePlant cell reports2019
Single and multiple gene knockouts by CRISPR-Cas9 in maize.
Article in Plant cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 68 citations in OpenAlex.
- Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants.Frontiers in plant science · 2020Pooled it
- Overexpression of the maize 9-lipoxygenase gene LOX4 confers resistance to Fusarium verticillioides via the oxylipin- and jasmonic acid-mediated pathways.Journal of experimental botany · 2026Article
- A loss-of-function of ZmWRKY125 induced by CRISPR/Cas9 improves resistance against Fusarium verticillioides in maize kernels.Plant cell reports · 2025Article
- Genome editing in maize and sorghum: A comprehensive review of CRISPR/Cas9 and emerging technologies.The plant genome · 2025Review
- Integrating Genetic Diversity and Agronomic Innovations for Climate-Resilient Maize Systems.Plants (Basel, Switzerland) · 2025Review
- Efficient mutagenesis and genotyping of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR.Plant methods · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants.Nature communications · 2023Article
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
- BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize.The Plant cell · 2023Article
- General guidelines for CRISPR/Cas-based genome editing in plants.Molecular biology reports · 2022Review
- Back to the wild: mining maize (Zea mays L.) disease resistance using advanced breeding tools.Molecular biology reports · 2022Article
- Function Analysis of theInternational journal of molecular sciences · 2022Article
- Natural and artificial sources of genetic variation used in crop breeding: A baseline comparator for genome editing.Frontiers in genome editing · 2022Review
- Evaluation of genome and base editing tools in maize protoplasts.Frontiers in plant science · 2022Article
- CRISPR-Cas technology opens a new era for the creation of novel maize germplasms.Frontiers in plant science · 2022Review
- Efficient virus-mediated genome editing in cotton using the CRISPR/Cas9 system.Frontiers in plant science · 2022Article
- The Functional Association ofInternational journal of molecular sciences · 2021Article
- Lipid anchoring and electrostatic interactions target NOT-LIKE-DAD to pollen endo-plasma membrane.The Journal of cell biology · 2021Article
- Genome editing in cereal crops: an overview.Transgenic research · 2021Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
Abstract
key messageThe analysis of 93 mutant alleles in 18 genes demonstrated that CRISPR-Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of single and multiple knockouts. CRISPR-Cas9 technology is a simple and efficient tool for targeted mutagenesis of the genome. It has been implemented in many plant species, including crops such as maize. Here we report single- and multiple-gene mutagenesis via stably transformed maize plants. Two different CRISPR-Cas9 vectors were used allowing the expression of multiple guide RNAs and different strategies to knockout either independent or paralogous genes. A total of 12 plasmids, representing 28 different single guide RNAs (sgRNAs), were generated to target 20 genes. For 18 of these genes, at least one mutant allele was obtained, while two genes were recalcitrant to sequence editing. 19% (16/83) of mutant plants showed biallelic mutations. Small insertions or deletions of less than ten nucleotides were most frequently observed, regardless of whether the gene was targeted by one or more sgRNAs. Deletions of defined regions located between the target sites of two guide RNAs were also reported although the exact deletion size was variable. Double and triple mutants were created in a single step, which is especially valuable for functional analysis of genes with strong genetic linkage. Off-target effects were theoretically limited due to rigorous sgRNA design and random experimental checks at three potential off-target sites did not reveal any editing. Sanger chromatograms allowed to unambiguously class the primary transformants; the majority (85%) were fully edited plants transmitting systematically all detected mutations to the next generation, generally following Mendelian segregation.
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Registered trials
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