ArticleCell death and differentiation2019
Reconstitution of male germline cell specification from mouse embryonic stem cells using defined factors in vitro.
Article in Cell death and differentiation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Molecular genetics of meiotic initiation in mammals.Biology of reproduction · 2026Article
- Induction of male neogametogenesis in a three-dimensional microenvironment supporting successful fertilization and proper embryo development.Molecular human reproduction · 2026Article
- Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026Review
- Establishment and optimization of the two-step induction system for generating primordial germ cell-like cells from chicken embryonic stem cells.FEBS open bio · 2026Article
- In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential.Molecular reproduction and development · 2025Review
- Bovine Peripheral Blood-Derived Mesenchymal Stem Cells (PB-MSCs) and Spermatogonial Stem Cells (SSCs) Display Contrasting Expression Patterns of Pluripotency and Germ Cell Markers under the Effect of Sertoli Cell Conditioned Medium.Animals : an open access journal from MDPI · 2024Article
- Livestock embryonic stem cells for reproductive biotechniques and genetic improvement.Animal reproduction · 2024Article
- Embryonic Stem Cells Can Generate Oral Epithelia under Matrix Instruction.International journal of molecular sciences · 2023Article
- Comparative Analysis of the Potential for Germ Cell (GC) Differentiation of Bovine Peripheral Blood Derived-Mesenchymal Stem Cells (PB-MSC) and Spermatogonial Stem Cells (SSC) in Co-Culture System with Sertoli Cells (SC).Animals : an open access journal from MDPI · 2023Article
- In vitro gametogenesis from embryonic stem cells in livestock species: recent advances, opportunities, and challenges to overcome.Journal of animal science · 2023Review
- Super-enhancers conserved within placental mammals maintain stem cell pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Integrated bioinformatics analysis of the transcription factor-mediated gene regulatory networks in the formation of spermatogonial stem cells.Frontiers in physiology · 2022Article
- BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2.Cell proliferation · 2021Article
- p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation.Stem cells international · 2021Article
- Article
- In Vitro Meiosis of Male Germline Stem Cells.Stem cell reports · 2020Article
- Characterization of porcine extraembryonic endoderm cells.Cell proliferation · 2019Article
- Generation of male germ cellsAsian journal of andrologyReview
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro induction of functional haploid cells from embryonic stem cells (ESCs) has been reported by several groups. However, these reports either involve complex induction process with undefined induction factors or show low-induction efficiency. Here, we report complete meiosis in vitro from ESCs with defined induction factors. ESCs were first induced into primordial germ cell-like cells, which were further induced into male germline cells, including spermatogonial stem cell-like cells (SSCLCs) and spermatid-like cells. Importantly, the obtained SSCLCs were functional as infertile male mice sired healthy offspring via SSCLC transplantation. Further, we found that eukaryotic translation initiation factor 2 subunit 3 and structural gene Y-linked (Eif2s3y) was essential for spermatogenesis. Eif2s3y-overexpressing ESCs showed enhanced spermatogenesis in vitro, as demonstrated by higher expression levels of SSC-specific markers during SSCLC induction process, improved reproductive ability recovery of infertile male mice, and increased efficiency of haploid cell induction. Our work provides a convenient and efficient approach to obtain functional male germline cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.