ArticleApplied and environmental microbiology2019
Changes in the Active, Dead, and Dormant Microbial Community Structure across a Pleistocene Permafrost Chronosequence.
Article in Applied and environmental microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- MATRIX: rapid quantification of total and active microbial cells with single-cell phenotypes for environmental microbiomes.mSystems · 2026Article
- Biogas production using the microbial community present in the soil from Deception Island, maritime Antarctica.Environmental science and pollution research international · 2026Article
- State-Specific Extraction of Environmental DNA: Spike-and-Recovery Controls to Validate and Optimise Extraction Protocols.Environmental microbiology · 2025Article
- The silent microbial shift: climate change amplifies pathogen evolution, microbiome dysbiosis, and antimicrobial resistance.Tropical diseases, travel medicine and vaccines · 2025Review
- Seasonally and niche-differentiated diversity of active, dormant and dead microbes in coastal waters and surface sediments.Communications biology · 2025Article
- Review
- Microbial and chemical predictors of methane release from a stratified thermokarst permafrost hotspot.Frontiers in microbiology · 2025Article
- Redox determines greenhouse gas production kinetics and metabolic traits in water-saturated thawing permafrost peat.ISME communications · 2025Article
- Consistent microbial responses during the aerobic thaw of Alaskan permafrost soils.Frontiers in microbiology · 2025Article
- Comparative analysis of prokaryotic microbiomes in high-altitude active layer soils: insights from Ladakh and global analogues using In-Silico approaches.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- On-Site Inactivation for Disinfection of Antibiotic-Resistant Bacteria in Hospital Effluent by UV and UV-LED.Antibiotics (Basel, Switzerland) · 2024Article
- Metagenomic insights into microbial community structure and metabolism in alpine permafrost on the Tibetan Plateau.Nature communications · 2024Article
- Microbial life in slow and stopped lanes.Trends in microbiology · 2024Review
- Fecal microbiota transplantation: current challenges and future landscapes.Clinical microbiology reviews · 2024Review
- Abrupt permafrost thaw triggers activity of copiotrophs and microbiome predators.FEMS microbiology ecology · 2023Article
- Earth to Mars: A Protocol for Characterizing Permafrost in the Context of Climate Change as an Analog for Extraplanetary Exploration.Astrobiology · 2023Article
- Permafrost microbial communities and functional genes are structured by latitudinal and soil geochemical gradients.The ISME journal · 2023Article
- Distinct taxonomic and functional profiles of high Arctic and alpine permafrost-affected soil microbiomes.Environmental microbiome · 2023Article
- RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities.Microbiome · 2023Article
- Microbial life in 25-m-deep boreholes in ancient permafrost illuminated by metagenomics.Environmental microbiome · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Permafrost hosts a community of microorganisms that survive and reproduce for millennia despite extreme environmental conditions, such as water stress, subzero temperatures, high salinity, and low nutrient availability. Many studies focused on permafrost microbial community composition use DNA-based methods, such as metagenomics and 16S rRNA gene sequencing. However, these methods do not distinguish among active, dead, and dormant cells. This is of particular concern in ancient permafrost, where constant subzero temperatures preserve DNA from dead organisms and dormancy may be a common survival strategy. To circumvent this, we applied (i) LIVE/DEAD differential staining coupled with microscopy, (ii) endospore enrichment, and (iii) selective depletion of DNA from dead cells to permafrost microbial communities across a Pleistocene permafrost chronosequence (19,000, 27,000, and 33,000 years old). Cell counts and analysis of 16S rRNA gene amplicons from live, dead, and dormant cells revealed how communities differ between these pools, how they are influenced by soil physicochemical properties, and whether they change over geologic time. We found evidence that cells capable of forming endospores are not necessarily dormant and that members of the class
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