ArticleParasites & vectors2019
Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis.
Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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14 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genotype distribution of Acanthamoeba in keratitis: a systematic review.Parasitology research · 2021Pooled it
- Prevalence of HSV Genomic Signatures Among Acanthamoeba Hosts and Contaminated Lens Cases: A Molecular and Clinical Study.Investigative ophthalmology & visual science · 2025Article
- Learning from the rDNA Operon: A Reanalysis of theMicroorganisms · 2024Article
- Molecular evidence of Monocercomonas and Acanthamoeba in the feces of captive reptiles.Parasitology research · 2022Article
- Assessment of genotypes, endosymbionts and clinical characteristics of Acanthamoeba recovered from ocular infection.BMC infectious diseases · 2022Article
- Transient internalization ofMicrobiology (Reading, England) · 2022Article
- Detection of Acanthamoeba spp. using carboxylesterase antibody and its usage for diagnosing Acanthamoeba-keratitis.PloS one · 2022Article
- Molecular Identification of Pathogenic Free-Living Amoeba from Household Biofilm Samples in Iran: A Risk Factor forMicroorganisms · 2021Article
- Potential anti-Heliyon · 2021Article
- Curcuma longa rhizome extract and Curcumin reduce the adhesion of Acanthamoeba triangularis trophozoites and cysts in polystyrene plastic surface and contact lens.International journal for parasitology. Drugs and drug resistance · 2020Article
- Acanthamoeba species isolated from Philippine freshwater systems: epidemiological and molecular aspects.Parasitology research · 2020Article
- Free-living amoebae and their relationship to air quality in hospital environments: characterization ofParasitology · 2020Article
- Article
- A descriptive case of persistentAccess microbiology · 2020Article
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Abstract
backgroundAcanthamoeba spp. are the causative agents of a severe keratitis occurring mainly in contact lens wearers. The genus comprises more than 24 species that are currently divided into 20 different genotypes (T1-T20) according to sequence variations in the 18S rRNA gene. The objective of this study was to identify the genotypes and sub-genotypes of Acanthamoeba isolates collected at the Parasitology Laboratory of the Public Health Institute of Chile, the only laboratory in the country where Acanthamoeba screening is performed. This is the first report of genotypic identification of clinical isolates of Acanthamoeba in Chile and one of the few in South America.
resultsIn this study, 114 Acanthamoeba isolates from 76 Acanthamoeba keratitis patients, obtained between 2005-2016, were genotyped. T4 was the predominant genotype; T2 and T11 genotypes, which are scarcely reported worldwide, were also identified in Chilean patients (one and two patients, respectively). This is the first report of T2 and T11 genotypes isolated from Acanthamoeba keratitis patients in South America. It is also the first report of the T2 genotype circulating in this continent. Analysis of the diagnostic fragment 3 region of the 18S rRNA gene showed 24 T4 variants, with a predominance of the sub-genotype T4/A, followed by T4/B, T4/G, T4/C and T4/D. Bayesian analysis revealed three groups among the T4 variants: two well supported groups that included 12 and 7 sub-genotypes, respectively, and a weakly supported group that included 5 sub-genotypes. Most of the predominant T4 sub-genotypes belonged to the same group, which included 71.3% of the patients, while some minority variants lied mainly in the other two clusters.
conclusionsT2, T4 and T11 genotypes were predominantly isolated from the Acanthamoeba keratitis patients in Chile. Chilean predominant T4 sub-genotypes, which have also been reported worldwide, formed a separate cluster of the minority T4 variants. This study provides useful information about the predominant genotypes and subgenotypes that would be useful in selecting suitable strains to develop immunological and/or molecular diagnostic assays in Chile.
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