ArticleBMC genomics2019
RNA-sequencing analysis of shell gland shows differences in gene expression profile at two time-points of eggshell formation in laying chickens.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Genomic analysis of the Ixworth chicken: insights into a local dual-purpose breed.BMC genomics · 2026Article
- Research Progress on Genetic Factors of Poultry Egg Quality: A Review.Animals : an open access journal from MDPI · 2025Review
- Regulatory effects of hawthorn leaf flavonoids and stevioside on the uterine function and eggshell quality in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Mapping multitissue regulatory variants reveals a liver-centric coexpression network associated with duck egg-laying performance.Genome research · 2025Article
- Dietary cholecalciferol and 25-hydroxyvitamin DAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
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- Plasma metabolomics of Mycoplasma synoviae infection in SPF White Leghorn hens by liquid chromatography-tandem mass spectrometry.Veterinary research · 2025Article
- Organic Trace Elements Improve the Eggshell Quality via Eggshell Formation Regulation during the Late Phase of the Laying Cycle.Animals : an open access journal from MDPI · 2024Article
- Transcriptome profiling analysis of uterus during chicken laying periods.BMC genomics · 2023Article
- Molecular Mechanism of m6A Methylation Modification GenesInternational journal of molecular sciences · 2023Article
- Effects of Selenium Yeast on Egg Quality, Plasma Antioxidants, Selenium Deposition and Eggshell Formation in Aged Laying Hens.Animals : an open access journal from MDPI · 2023Article
- Circadian miR-218-5p targets gene CA2 to regulate uterine carbonic anhydrase activity during egg shell calcification.Poultry science · 2022Article
- Low Level of Dietary Organic Trace Minerals Improved Egg Quality and Modulated the Status of Eggshell Gland and Intestinal Microflora of Laying Hens During the Late Production Stage.Frontiers in veterinary science · 2022Article
- RNA Sequencing of the Pituitary Gland and Association Analyses RevealFrontiers in veterinary science · 2022Article
- Transcriptome profiling in response to Kanamycin B reveals its wider non-antibiotic cellular function inFrontiers in microbiology · 2022Article
- Multiomic analysis revealed the regulatory role of theFrontiers in genetics · 2022Article
- Cuticle deposition duration in the uterus is correlated with eggshell cuticle quality in White Leghorn laying hens.Scientific reports · 2021Article
- Uterine transcriptome analysis reveals mRNA expression changes associated with the ultrastructure differences of eggshell in young and aged laying hens.BMC genomics · 2020Article
- Transcriptome analysis of the uterus of hens laying eggs differing in cuticle deposition.BMC genomics · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundEggshell formation takes place in the shell gland of the oviduct of laying hens. The eggshell is rich in calcium and various glycoproteins synthesised in the shell gland. Although studies have identified genes involved in eggshell formation, little is known about the regulation of genes in the shell gland particularly in a temporal manner. The current study investigated the global gene expression profile of the shell gland of laying hens at different time-points of eggshell formation using RNA-Sequencing (RNA-Seq) analysis.
resultsGene expression profiles of the shell gland tissue at 5 and 15 h time-points were clearly distinct from each other. Out of the 14,334 genes assessed for differential expression in the shell gland tissue, 278 genes were significantly down-regulated (log
conclusionsThe down-regulated genes at 15 h relative to the 5 h time-point were most likely involved in the transport of molecules and synthesis activities, initiating the formation of the eggshell. The up-regulated genes were most likely involved in calcium transportation, as well as synthesis and secretory activities of ions and molecules, reflecting the peak stage of eggshell formation. The findings in the current study improve our understanding of eggshell formation at the molecular level and provide a foundation for further studies of mRNA and possibly microRNA regulation involved in eggshell formation in the shell gland of laying hens.
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