ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2019
Characterization of Xenopus laevis guanine deaminase reveals new insights for its expression and function in the embryonic kidney.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 5 citations in OpenAlex.
- Iron Deficiency Impairs Mitochondrial Energetics and Early Axonal Growth and Branching in Developing Hippocampal Neurons.The Journal of nutrition · 2026Article
- Characterization of Guanine Deaminase fromFoods (Basel, Switzerland) · 2025Article
- Article
- Real-Time Monitoring of Human Guanine Deaminase Activity by an Emissive Guanine Analog.ACS chemical biology · 2021Article
- Regulation of MT dynamics via direct binding of an Abl family kinase.The Journal of cell biology · 2019Article
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7 authors at 1 institution in 1 country.
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Abstract
backgroundThe mammalian guanine deaminase (GDA), called cypin, is important for proper neural development, by regulating dendritic arborization through modulation of microtubule (MT) dynamics. Additionally, cypin can promote MT assembly in vitro. However, it has never been tested whether cypin (or other GDA orthologs) binds to MTs or modulates MT dynamics. Here, we address these questions and characterize Xenopus laevis GDA (Gda) for the first time during embryonic development.
resultsWe find that exogenously expressed human cypin and Gda both display a cytosolic distribution in primary embryonic cells. Furthermore, while expression of human cypin can promote MT polymerization, Xenopus Gda has no effect. Additionally, we find that the tubulin-binding collapsin response mediator protein (CRMP) homology domain is only partially conserved between cypin and Gda. This likely explains the divergence in function, as we discovered that the cypin region containing the CRMP homology and PDZ-binding domain is necessary for regulating MT dynamics. Finally, we observed that gda is strongly expressed in the kidneys during late embryonic development, although it does not appear to be critical for kidney development.
conclusionsTogether, these results suggest that GDA has diverged in function between mammals and amphibians, and that mammalian GDA plays an indirect role in regulating MT dynamics. Developmental Dynamics 248:296-305, 2019. © 2019 Wiley Periodicals, Inc.
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