Evidence map›Paper›PMID 30680681›Full record

ArticleSleep & breathing = Schlaf & Atmung2019

Blocking C/EBP β protects vascular endothelial cells from injury induced by intermittent hypoxia.

Yu Feng, Qingchu Li, Yinxiang Wu, Nana Zhao, Lu Li, Li Li, Liming Zhao

Abstract read
PubMed Publisher
In one paragraph

Article in Sleep & breathing = Schlaf & Atmung, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 6 citations in OpenAlex.

  1. Positive feedback loop between NRASBiochemical and biophysical research communications · 2025
    Article
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  5. Article
  6. CCAAT/Enhancer-Binding ProteinJournal of diabetes research · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yu Feng *Department of Respiratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Pudong, Shanghai, 200120, China.
Qingchu Li *Department of Radiology, Secondary Affiliated Hospital of the Second Military Medical University, Shanghai, 200001, China.
Yinxiang WuDepartment of Respiratory Medicine, Third Affiliated Hospital of Second Military Medical University, Shanghai, 200438, China.
Nana ZhaoDepartment of Respiratory Medicine, Third Affiliated Hospital of Second Military Medical University, Shanghai, 200438, China.
Lu LiDepartment of Respiratory Medicine, Third Affiliated Hospital of Second Military Medical University, Shanghai, 200438, China.
Li LiDepartment of Teaching Support, Second Military Medical University, Shanghai, 200433, China. czjwlili@163.com.
Liming ZhaoDepartment of Respiratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Pudong, Shanghai, 200120, China. zhao.liming@hotmail.com.ORCID http://orcid.org/0000-0003-2778-8793
Second Military Medical University · CNShanghai East Hospital · CN

Funding

National Natural Sciences Fund Project of China 81372529
6 · The paper itself

Abstract

backgroundIntermittent hypoxia (IH) can damage endothelial cells and lead to apoptosis in obstructive sleep apnea-hypopnea syndrome (OSAHS). Hypoxia induces apoptosis in endothelial cells via upregulation of endothelin-1 (ET-1) and hypoxia inducible factor-1 alpha (HIF-1α) plays a key role in the hypoxic stress response. PURPOSE: We investigated an approach to diminish the negative effect of HIF-1α while maintaining its protective effect.

methodsHuman umbilical vein endothelial cells (HUVECs) were subjected to sustained hypoxia (SH) or IH for 24 h, and the responses of HIF-1α, CCAAT/enhancer binding protein beta (C/EBP β), and endothelin-1 (ET-1) were assessed by western blotting. A luciferase reporter system was employed to verify the potential binding site (transcription factor binding site, TFBS) for C/EBP β in the ET-1 promoter. The specificity of regulation of ET-1 by HIF-1α via C/EBP β was evaluated by a lentiviral system. The effects of silencing of C/EBP β on IH-induced apoptosis, vascular endothelial growth factor (VEGF) protein levels, proliferation, and in vitro tube formation were studied.

resultsWe found that IH significantly increased HIF-1α, C/EBP β, and ET-1 in HUVECs. Knockdown of HIF-1α or C/EBP β inhibited the upregulation of ET-1 induced by IH. Blocking C/EBP β impaired IH-induced apoptosis but did not affect VEGF expression, proliferation, or in vitro tube formation. C/EBP β was shown to mediate increased ET-1 transcription by HIF-1α through the TFBS, 5'-GTTGCCTGTTG-3', in ET-1 promoter.

conclusionSilencing of C/EBP β can suppress apoptosis but does not affect the protective role of HIF-1α in the hypoxic stress response.

Indexed as

ApoptosisCCAAT-Enhancer-Binding Protein-betaEndothelial CellsHumansHypoxia-Inducible Factor 1, alpha SubunitSleep Apnea, ObstructiveVascular Endothelial Growth Factor ACCAAT-Enhancer-Binding Protein-betaCEBPB protein, humanHIF1A protein, humanHypoxia-Inducible Factor 1, alpha SubunitVascular Endothelial Growth Factor AC/EBP βET-1HIF-1αIntermittent hypoxiaOSAHS

Identifiers

PMID30680681
OpenAlexW2911577345

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.