Evidence map›Paper›PMID 30680473›Full record

ArticleJournal of industrial microbiology & biotechnology2019

A selective genome-guided method for environmental Burkholderia isolation.

F P Jake Haeckl, João L Baldim, Dasha Iskakova, Kenji L Kurita, Marisi G Soares, Roger G Linington

Abstract read
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In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Dataset ofData in brief · 2026
    Article
  3. Article
  4. Article
  5. Methodological tools to study species of the genus Burkholderia.Applied microbiology and biotechnology · 2021
    Review
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

F P Jake HaecklDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
João L BaldimChemistry Institute of Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.ORCID http://orcid.org/0000-0002-2239-0113
Dasha IskakovaDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
Kenji L KuritaDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada.
Marisi G SoaresChemistry Institute of Federal University of Alfenas, Alfenas, Minas Gerais, Brazil.ORCID http://orcid.org/0000-0001-9221-9867
Roger G LiningtonDepartment of Chemistry, Simon Fraser University, Burnaby, BC, Canada. rliningt@sfu.ca.ORCID http://orcid.org/0000-0003-1818-4971

Funding

Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior 8074135Natural Sciences and Engineering Research Council of Canada RGPIN-2016-03962
6 · The paper itself

Abstract

The genus Burkholderia is an emerging source of novel natural products chemistry, yet to date few methods exist for the selective isolation of strains of this genus from the environment. More broadly, tools to efficiently design selection media for any given genus would be of significant value to the natural products and microbiology communities. Using a modification of the recently published SMART protocol, we have developed a two-stage isolation protocol for strains from the genus Burkholderia. This method uses a combination of selective agar isolation media and multiplexed PCR profiling to derive Burkholderia strains from environmental samples with 95% efficiency. Creation of this new method paves the way for the systematic exploration of natural products chemistry from this important genus and offers new insight into potential methods for selective isolation method development for other priority genera.

Indexed as

Biological ProductsBurkholderiaComputational BiologyCulture MediaDNA, BacterialGenome, BacterialPhylogenyReproducibility of ResultsRNA, Ribosomal, 16SBiological ProductsCulture MediaDNA, BacterialRNA, Ribosomal, 16SBurkholderiaNatural productsRhizomeSelective isolation

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.