An improved method for high-temperature induced embryo sac chromosome doubling in Populus simonii Carr. × P. nigra var. italica (Moench.) Kochne, an interspecific hybrid of Tacamahaca and Aigeiros poplars.

Wenting Xu, Liqin Guo, Yan Zhang, Jian Zhao, Zunzheng Wei, Jinfeng Zhang

Open access · goldAbstract read

In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.9field-weighted citation impact, top 18% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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1 citing paper in PubMed, 4 citations in OpenAlex.

Study on the differences of phyllosphere microorganisms between poplar hybrid offspring and their parents.PeerJ · 2022
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5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Wenting XuBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants biological engineering of State Forestry Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China.

Liqin GuoBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants biological engineering of State Forestry Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China.

Yan ZhangBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants biological engineering of State Forestry Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China.

Jian ZhaoBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants biological engineering of State Forestry Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China.

Zunzheng WeiBeijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing, 100091, P. R. China. weizunzheng@163.com.

Jinfeng ZhangBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Key Laboratory of Forest Trees and Ornamental Plants biological engineering of State Forestry Administration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, P. R. China. zjf@bjfu.edu.cn.

Beijing Forestry University · CNBeijing Academy of Agricultural and Forestry Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chromosome doubling is considered an important technique in poplar breeding, with many triploid clones being artificially induced and selected for promotion in the north and northeast of China because of their outstanding traits in vegetative growth and environmental adaption. In this study, the triploid yield of Populus simonii Carr × P. nigra var. italica (Moench.) Kochne was 23.41%, which exceeded the yield attained in our previous studies due to the use of an optimized method of chromosome doubling in the embryo sac at a high temperature. The development of the embryo sac after the pollination of this hybrid was investigated to determine the induction period. Ploidy of seedlings was identified by flow cytometry after initial filtering using the chloroplast counting method. Eleven triploids and one tetraploid were ultimately obtained, and the optimal operating conditions were exposure of female catkins to 41 °C for 2 h at 66 h after pollination (HAP). This study identified an efficient method of chromosome doubling in P. simonii × P. nigra var. italica and provided several polyploids for Populus polyploid breeding programs and subsequent studies.

Indexed as

Hot TemperatureChimeraChromosomes, PlantFlowersHybridization, GeneticPlant BreedingPollinationPopulusSeedlingsTetraploidyTriploidy

Identifiers

PMID30679816

DOI10.1038/s41598-018-37297-w

PMCPMC6346037

OpenAlexW2912320113

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.