ArticleScientific reports2019
Comparison of regeneration capacity and Agrobacterium-mediated cell transformation efficiency of different cultivars and rootstocks of Vitis spp. via organogenesis.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Reducing recalcitrance of black pepper toHorticulture research · 2026Article
- A Fruit-Pulp-Derived Callus-Level Agrobacterium-Mediated Transformation Platform forPlants (Basel, Switzerland) · 2026Article
- Overview on Current Selectable Marker Systems and Novel Marker Free Approaches in Fruit Tree Genetic Engineering.International journal of molecular sciences · 2024Review
- Organogenesis in a Broad Spectrum of Grape Genotypes andPlants (Basel, Switzerland) · 2024Article
- New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i.Plant methods · 2024Article
- Efficient protocol ofFrontiers in plant science · 2023Article
- Boosting grapevine breeding for climate-smart viticulture: from genetic resources to predictive genomics.Frontiers in plant science · 2023Review
- A Strategy for the Production and Molecular Validation of Agrobacterium-Mediated Intragenic Octoploid Strawberry.Plants (Basel, Switzerland) · 2021Article
- Creating a novel petal regeneration system for function identification of colour gene of grape hyacinth.Plant methods · 2021Article
- Biotechnological Approaches: Gene Overexpression, Gene Silencing, and Genome Editing to Control Fungal and Oomycete Diseases in Grapevine.International journal of molecular sciences · 2020Review
- Embryogenic callus induction andPlant biotechnology (Tokyo, Japan) · 2020Article
- Biosafety of GM Crop Plants Expressing dsRNA: Data Requirements and EU Regulatory Considerations.Frontiers in plant science · 2020Review
- Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Plants (Basel, Switzerland) · 2019Article
- Establishment of Efficient Genetic Transformation Systems and Application of CRISPR/Cas9 Genome Editing Technology inInternational journal of molecular sciences · 2019Article
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The success of in vitro plant regeneration and the competence of genetic transformation greatly depends on the genotype of the species of interest. In previous work, we developed a method for the efficient Agrobacterium-mediated genetic transformation via organogenesis of V. vinifera cultivar Thompson Seedless, by using meristematic bulk (MB) as starting tissue. In this study, we applied this method for the regeneration and transformation of MBs obtained from the Italian cultivar Ciliegiolo and two of the commonly used Vitis rootstocks, 110 Richter and Kober 5BB, in comparison with Thompson Seedless. The A. tumefaciens strain EHA105, harbouring pK7WG2 binary vector, was used for the transformation trials, which allowed selection through the enhanced-green fluorescent protein (eGFP) and the neomycin phosphotransferase (nptII) gene. Putative transformed tissues and/or shoots were identified by either a screening based on the eGFP expression alone or its use in combination with kanamycin in the medium. MBs obtained from Thompson Seedless showed the highest regeneration and transformation cell competence, which subsequently allowed the recovery of stably transformed plants. Ciliegiolo, 110 Richter, and Kober 5BB, produced actively growing transgenic calli showing eGFP fluorescence, more consistently on selective media, but had no regenerative competence.
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