ArticleScientific reports2019
Liposome-based transfection enhances RNAi and CRISPR-mediated mutagenesis in non-model nematode systems.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 65 citations in OpenAlex.
- Multiple losses of ecdysone receptor genes in nematodes: an alternative evolutionary scenario of molting regulation.Molecular biology and evolution · 2026Article
- Transgenesis and targeted mutagenesis in the human-parasitic nematodeFrontiers in parasitology · 2026Review
- CRISPR/Cas genome editing, functional genomics, and diagnostics for parasitic helminths.International journal for parasitology · 2025Review
- Identity and functions of monoaminergic neurons in the predatory nematodebioRxiv : the preprint server for biology · 2025Article
- Carbon dioxide shapes parasite-host interactions in a human-infective nematode.Current biology : CB · 2025Article
- Dafadine Does Not Promote Dauer Development inmicroPublication biology · 2025Article
- Microparticle Bombardment as a Method for Transgenesis inmicroPublication biology · 2025Article
- Systemic Immune Modulation by Gastrointestinal Nematodes.Annual review of immunology · 2024Review
- The contribution of an X chromosome QTL to non-Mendelian inheritance and unequal chromosomal segregation in Auanema freiburgense.Genetics · 2024Article
- Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematode Steinernema hermaphroditum.Genetics · 2024Article
- Invade or die: behaviours and biochemical mechanisms that drive skin penetration inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Astacin metalloproteases in human-parasitic nematodes.Advances in parasitology · 2024Review
- Redox-Responsive Polymeric Nanogels as Efficient mRNA Delivery Vehicles inmicroPublication biology · 2024Article
- Lentiviral Transduction-based CRISPR/Cas9 Editing ofCurrent genomics · 2023Article
- Unraveling effects of anti-aging drugs on C. elegans using liposomes.GeroScience · 2023Article
- CRISPR/Cas9 mediated gene editing in non-model nematodeFrontiers in genome editing · 2023Article
- A Decade of CRISPR-Cas Gnome Editing inInternational journal of molecular sciences · 2022Review
- The nematode Oscheius tipulae as a genetic model for programmed DNA elimination.Current biology : CB · 2022Article
- Using newly optimized genetic tools to probe Strongyloides sensory behaviors.Molecular and biochemical parasitology · 2022Review
- Sexual morph specialisation in a trioecious nematode balances opposing selective forces.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Nematodes belong to one of the most diverse animal phyla. However, functional genomic studies in nematodes, other than in a few species, have often been limited in their reliability and success. Here we report that by combining liposome-based technology with microinjection, we were able to establish a wide range of genomic techniques in the newly described nematode genus Auanema. The method also allowed heritable changes in dauer larvae of Auanema, despite the immaturity of the gonad at the time of the microinjection. As proof of concept for potential functional studies in other nematode species, we also induced RNAi in the free-living nematode Pristionchus pacificus and targeted the human parasite Strongyloides stercoralis.
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