ArticleScientific reports2019
Effects of the long-term storage of human fecal microbiota samples collected in RNAlater.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Towards a Standard Protocol for Fecal Microbiota Transplantation in Irritable Bowel Syndrome.Journal of neurogastroenterology and motility · 2026Review
- Impact of degradation and time of sampling on gut Microbiome composition in wild-caught marine fish.Scientific reports · 2025Article
- Building a foundation to promote Women's health research in Dubai. Study protocol for a project to investigate the feasibility of establishing a dedicated Women's health biobank.Frontiers in global women's health · 2025Article
- Assessment of the reproducibility of bacterial membrane vesicle isolation and characterization.Extracellular vesicles and circulating nucleic acids · 2025Article
- Chronic Rhinosinusitis-Microbiological Etiology, Potential Genetic Markers, and Diagnosis.International journal of molecular sciences · 2024Review
- Evaluation of fecal sample collection methods for feline gut microbiome profiling: fecal loop vs. litter box.Frontiers in microbiology · 2024Article
- Article
- Effects of storage conditions on the microbiota of fecal samples collected from dairy cattle.PloS one · 2024Article
- Methodological considerations for aqueous environmental RNA collection, preservation, and extraction.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Article
- From-Toilet-to-Freezer: A Review on Requirements for an Automatic Protocol to Collect and Store Human Fecal Samples for Research Purposes.Biomedicines · 2023Review
- Salivary level of microRNA-146a and microRNA-155 biomarkers in patients with oral lichen planus versus oral squamous cell carcinoma.BMC oral health · 2023Article
- Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiome.Scientific reports · 2023Article
- Stability of the Fecal and Oral Microbiome over 2 Years at -80°C for Multiple Collection Methods.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2023Article
- Long-term taxonomic and functional stability of the gut microbiome from human fecal samples.Scientific reports · 2023Article
- Compositional changes in fecal microbiota associated with clinical phenotypes and prognosis in Korean patients with inflammatory bowel disease.Intestinal research · 2023Article
- Compositional Changes in the Gut Microbiota of Responders and Non-responders to Probiotic Treatment Among Patients With Diarrhea-predominant Irritable Bowel Syndrome: A Post Hoc Analysis of a Randomized Clinical Trial.Journal of neurogastroenterology and motility · 2022Article
- Effects of Preservation and Propagation Methodology on Microcosms Derived from the Oral Microbiome.Microorganisms · 2022Article
- Seasonal and geographical differences in the ruminal microbial and chloroplast composition of sika deer (Cervus nippon) in Japan.Scientific reports · 2022Article
- Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies.Journal of animal science · 2022Review
- Comparison of Fecal Sample Collection Methods for Microbial Analysis Embedded within Colorectal Cancer Screening Programs.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2022Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adequate storage of fecal samples from clinical trials is crucial if analyses are to be performed later and in long-term studies. However, it is unknown whether the composition of the microbiota is preserved during long-term stool storage (>1 year). We therefore evaluated the influence of long-term storage on the microbiota composition of human stool samples collected in RNAlater and stored for approximately five years at -80 °C. We compared storage effects on stool samples from 24 subjects with the effects of technical variation due to different sequencing runs and biological variation (intra- and inter-subject), in another 101 subjects, based on alpha-diversity, beta-diversity and taxonomic composition. We also evaluated the impact of initial alpha-diversity and fecal microbiota composition on beta-diversity instability upon storage. Overall, long-term stool storage at -80 °C had only limited effects on the microbiota composition of human feces. The magnitude of changes in alpha- and beta- diversity and taxonomic composition after long-term storage was similar to inter-sequencing variation and smaller than biological variation (both intra- and inter-subject). The likelihood of fecal samples being affected by long-term storage correlated with the initial relative abundance of some genera and tend to be affected by initial taxonomic richness.
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