ArticleScientific reports2019
Can enzyme proximity accelerate cascade reactions?
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Catalyst-free single-molecule cascade photoreactions for spatiotemporally programmed encryption.Nature communications · 2026Article
- Complete enzyme clustering enhances coenzyme Q biosynthesis via substrate channeling.Nature communications · 2026Article
- Hierarchically Engineered Multi-Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Dynamic Hydrogel-Based Cytomimetic Models for Chemical Information Conduction.Small science · 2025Article
- Membrane nanodomains to shape plant cellular functions and signaling.The New phytologist · 2025Review
- Size Sensitivity of Metabolite Diffusion in Macromolecular Crowds.Nano letters · 2024Article
- Review
- Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis.Metabolic engineering communications · 2023Article
- From Protein Film Electrochemistry to Nanoconfined Enzyme Cascades and the Electrochemical Leaf.Chemical reviews · 2023Review
- Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades.Nature communications · 2023Article
- Mechanistic Aspects for the Modulation of Enzyme Reactions on the DNA Scaffold.Molecules (Basel, Switzerland) · 2022Review
- Article
- Programmable protein topology via SpyCatcher-SpyTag chemistry in one-pot cell-free expression system.Protein science : a publication of the Protein Society · 2022Article
- Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Genetic fusion of P450 BM3 and formate dehydrogenase towards self-sufficient biocatalysts with enhanced activity.Scientific reports · 2021Article
- Metabolic channeling: predictions, deductions, and evidence.Molecular cell · 2021Review
- Synthetic Biology towards Improved Flavonoid Pharmacokinetics.Biomolecules · 2021Review
- Cluster Channeling in Cascade Reactions.The journal of physical chemistry. B · 2021Article
- The power of electrified nanoconfinement for energising, controlling and observing long enzyme cascades.Nature communications · 2021Article
- Channeling a complex question.Nature chemical biology · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The last decade has seen an exponential expansion of interest in conjugating multiple enzymes of cascades in close proximity to each other, with the overarching goal being to accelerate the overall reaction rate. However, some evidence has emerged that there is no effect of proximity channeling on the reaction velocity of the popular GOx-HRP cascade, particularly in the presence of a competing enzyme (catalase). Herein, we rationalize these experimental results quantitatively. We show that, in general, proximity channeling can enhance reaction velocity in the presence of competing enzymes, but in steady state a significant enhancement can only be achieved for diffusion-limited reactions or at high concentrations of competing enzymes. We provide simple equations to estimate the effect of channeling quantitatively and demonstrate that proximity can have a more pronounced effect under crowding conditions in vivo, particularly that crowding can enhance the overall rates of channeled cascade reactions.
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Registered trials
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