ArticleScientific reports2019
A cationic near infrared fluorescent agent and ethyl-cinnamate tissue clearing protocol for vascular staining and imaging.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Innovative Technologies for Kidney Research: Three-Dimensional Imaging and Quantification.Seminars in nephrology · 2026Review
- Mapping the arterial vascular network in an intact human kidney using hierarchical phase-contrast tomography.Npj imaging · 2025Article
- Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.Chemical & biomedical imaging · 2024Article
- Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy.Scientific reports · 2024Article
- Mapping the blood vasculature in an intact human kidney using hierarchical phase-contrast tomography.bioRxiv : the preprint server for biology · 2024Article
- Extracellular vesicles in kidney development and pediatric kidney diseases.Pediatric nephrology (Berlin, Germany) · 2024Review
- Beyond 2D: A scalable and highly sensitive method for a comprehensive 3D analysis of kidney biopsy tissue.PNAS nexus · 2024Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- Sensory innervation in the prostate and a role for calcitonin gene-related peptide in prostatic epithelial proliferation.Frontiers in molecular neuroscience · 2024Article
- Microporous Polylactic Acid Scaffolds Enable Fluorescence-Based Perfusion Imaging of Intrinsic In Vivo Vascularization.International journal of molecular sciences · 2023Article
- Extracellular vesicles in kidney disease.Nature reviews. Nephrology · 2022Review
- Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies.Histochemistry and cell biology · 2022Review
- Article
- A simple optical tissue clearing pipeline for 3D vasculature imaging of the mediastinal organs in mice.International journal of experimental pathology · 2021Article
- Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?International journal of molecular sciences · 2021Review
- Review
- Mapping vascular and glomerular pathology in a rabbit model of neonatal acute kidney injury using MRI.Anatomical record (Hoboken, N.J. : 2007) · 2020Article
- Interrogating Cellular Communication in Cancer with Genetically Encoded Imaging Reporters.Radiology. Imaging cancer · 2020Review
- Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- Efficient Tissue Clearing and Multi-Organ Volumetric Imaging Enable Quantitative Visualization of Sparse Immune Cell Populations During Inflammation.Frontiers in immunology · 2020Article
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7 authors.
Funding
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Abstract
Understanding vascular structures and dysfunction is a fundamental challenge. This task has been approached by using traditional methodologies such as microscopic computed tomography and magnetic resonance imaging. Both techniques are not only expensive but also time-consuming. Here, we present a new method for visualizing vascular structures in different organs in an efficient manner. A cationic near infrared (NIR) fluorescent dye was developed with attractive features to specifically stain blood vessels. Furthermore, we refined the process of organ staining and harvesting by retrograde perfusion and optimized the subsequent dehydration and clearing process by the use of an automatic tissue processor and a non-toxic substance, ethyl-cinnamate. Using this approach, the time interval between organ harvesting and microscopic analysis can be reduced from day(s) or weeks to 4 hours. Finally, we have demonstrated that the new NIR fluorescent agent in combination with confocal or light-sheet microscopy can be efficiently used for visualization of vascular structures, such as the blood vessels in different organs e.g. glomeruli in kidneys, with an extremely high resolution. Our approach facilitates the development of automatic image processing and the quantitative analysis to study vascular and kidney diseases.
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