ArticleScientific reports2019
The proteome of frozen-thawed pig spermatozoa is dependent on the ejaculate fraction source.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Frozen Boar Semen Can Be Successfully Stored at -80°C, Reducing Need for LNReproduction in domestic animals = Zuchthygiene · 2026Article
- Modulation of NETosis in Swine Neutrophil-Spermatozoa Co-Cultures In Vitro: Effects of Butylated Hydroxytoluene, Albumin, Prostaglandin EAntioxidants (Basel, Switzerland) · 2025Article
- Article
- Techniques to Determine Mammalian Sperm Capacitation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Comparative Study on the Sperm Proteomes of Horses and Donkeys.Animals : an open access journal from MDPI · 2024Article
- Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction.Journal of animal science and biotechnology · 2023Article
- Type I Diabetes in Zebrafish Reduces Sperm Quality and Increases Insulin and Glucose Transporter Transcripts.International journal of molecular sciences · 2023Article
- Altered ureido protein modification profiles in seminal plasma extracellular vesicles of non-normozoospermic men.Frontiers in endocrinology · 2023Article
- iTRAQ-based proteomic analysis of sperm reveals candidate proteins that affect the quality of spermatozoa from boars on plateaus.Proteome science · 2021Article
- Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.Frontiers in cell and developmental biology · 2021Article
- Cryopreservation Differentially Alters the Proteome of Epididymal and Ejaculated Pig Spermatozoa.International journal of molecular sciences · 2019Article
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7 authors.
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Abstract
The preservation of sperm functional parameters and fertility post-cryopreservation largely varies in the porcine, a species with a fractionated ejaculate. Although intrinsic individual differences have primarily been linked to this variation, differences in protein abundance among frozen-thawed (FT)-spermatozoa are far more relevant. This study, performed in two experiments, looked for proteomic quantitative differences between FT-sperm samples differing in post-thaw viability, motility, apoptosis, membrane lipid peroxidation and nuclear DNA fragmentation. The spermatozoa were either derived from the sperm-rich ejaculate fraction (SRF) or the entire ejaculate (Experiment 1) or from the first 10 mL of the SRF, the remaining SRF and the post-SRF (Experiment 2). Quantitative sperm proteomic differences were analysed using a LC-ESI-MS/MS-based SWATH approach. In Experiment 1, FT-spermatozoa from the SRF showed better preservation parameters than those from the entire ejaculate, with 26 Sus scrofa proteins with functional sperm relevance showing relative quantitative differences (FC ≥ 1.5) between sperm sources. In Experiment 2, FT-spermatozoa from the first 10 mL of the SRF and the remaining SRF were qualitatively better than those from the post-SRF, and 187 proteins showed relative quantitative differences among the three ejaculate sources. The results indicate that quantitative proteome differences are linked to sperm cryosurvival.
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